Strain differences in Blastocystis isolates as detected by a single set of polymerase chain reaction primers

Init, I.; Mak, J.W.; Hakim, S.L.; Yong, H.S. (1999) Strain differences in Blastocystis isolates as detected by a single set of polymerase chain reaction primers. Parasitology Research, 85 (2). pp. 131-134. ISSN 0044-3255

[img] PDF - Published Version
Restricted to Repository staff only [error in script]

Download (99Kb) | Request a copy

    Abstract

    A total of 20 isolates of Blastocystis were characterized using a single set of polymerase chain reaction (PCR) primers. The amplification product revealed five types of pattern. All four isolates from Singapore yielded PCR products quite different from those of the local isolates. However, most of the local isolates showed a major product at either 280 or 500 bp, or both. We also suspected that the amplification product detected at 280 bp might be an indicator of the pathogenicity of this parasite. One isolate (M12) obtained from a monkey showed patterns similar to those of human isolates (10203 and KP1) and probably belongs to the same strain. The results indicate that the intraspecific or interstrain variations in these 20 Blastocystis isolates belong to 5 different patterns. The differences among isolates of the same strain revealed by the presence or absence of certain amplification products showed further intrastrain variations in this parasite.

    Item Type: Article
    Creators:
    1. Init, I.( Department of Parasitology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    2. Mak, J.W.
    3. Hakim, S.L.
    4. Yong, H.S.
    Journal or Publication Title: Parasitology Research
    Additional Information: Init, I Mak, JW Hakim, SL Yong, HS
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Mr Jenal S
    Date Deposited: 10 Dec 2012 10:41
    Last Modified: 10 Dec 2012 10:41
    URI: http://eprints.um.edu.my/id/eprint/4119

    Actions (For repository staff only: Login required)

    View Item

    Document Downloads

    More statistics for this item...