Efficacy of entomopathogenic fungal formulations against Elasmolomus pallens (Dallas) (Hemiptera: Rhyparochromidae) and their extracellular enzymatic activities

Umaru, Fredrick Fidelis and Simarani, Khanom (2022) Efficacy of entomopathogenic fungal formulations against Elasmolomus pallens (Dallas) (Hemiptera: Rhyparochromidae) and their extracellular enzymatic activities. Toxins, 14 (9). ISSN 2072-6651, DOI https://doi.org/10.3390/toxins14090584.

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Abstract

Elasmolomus pallens are post-harvest insect pests of peanuts that are becoming resistant to chemical insecticides. In this, we study evaluated the effect of conidial formulations on entomopathogenic fungi against E. pallens to reduce the adverse effects. Fungal conidia were formulated and applied on sterile filter papers at varying concentrations (1 x 10(4)-1 x 10(8) conidia mL(-1)) inside plastic containers. The test insects were exposed and maintained in a relative humidity of 80 +/- 10% for 10 d at room temperature (25 +/- 2 degrees C). Mortality was recorded every 24 h. Dose-response bioassay (LC50 and LC90) values for Aspergillus flavus formulated in oil were 1.95 x 10(6) and 3.66 x 10(9) conidia/mL, whereas formulations in Tween 80 had 9.36 x 10(7) and 6.50 x 10(9) conidia/mL. However, oil-formulated Metarhizium anisopliae had 3.92 x 10(6) and 2.57 x 10(8) conidia/mL, with 6.85 x 10(6) and 5.37 x 10(8), for formulations in Tween 80. A. flavus had LT50 values of 3.3 and 6.6 days, whereas M. anisopliae had LT50 values of 3.6 and 5.7 d. Maximum protease, chitinase, and lipase activities of 2.51, 0.98, and 3.22 U/mL, respectively, were recorded for A. flavus, whereas values of 2.43, 0.93, and 3.46 were recorded for M. anisopliae. The investigated pathogens demonstrate potential against E. pallens; therefore, their applicability under field conditions requires further investigation.

Item Type: Article
Funders: University of Malaya Center of Research Grant Management (PPGP) [PG343 2016A]
Uncontrolled Keywords: Biological control; Bioassay; Enzyme; Entomopathogenic fungi; Oil formulation; Seed bug
Subjects: Q Science > Q Science (General)
Q Science > QK Botany
Divisions: Faculty of Science
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 11 Sep 2023 03:30
Last Modified: 11 Sep 2023 03:30
URI: http://eprints.um.edu.my/id/eprint/41156

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