Molecular detection of Giardia contamination in water bodies in a zoo

Lim, Y.A.; Lai, M.M.; Mahdy, M.A.; Mat Naim, H.R.; Smith, H.V. (2009) Molecular detection of Giardia contamination in water bodies in a zoo. Environ Res, 109 (7). pp. 857-9. ISSN 1096-0953

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    Abstract

    We used a combined microscopy-molecular approach to determine the occurrence and identities of waterborne Giardia sp. cysts isolated from 18 separate, 10l grab samples collected from a Malaysian zoo. Microscopy revealed that 17 of 18 samples were Giardia cyst positive with concentrations ranging from 1 to 120 cysts/l. Nine (52.9) of the 17 cyst positive samples produced amplicons of which 7 (77.8) could be sequenced. Giardia duodenalis assemblage A (6 of 7) and assemblage B (1 of 7), both infectious to humans, were identified at all sampling sites at the zoo. The presence of human infectious cysts raises public health issues, and their occurrence, abundance and sources should be investigated further. In this zoo setting, our data highlight the importance of incorporating environmental sampling (monitoring) in addition to routine faecal examinations to determine veterinary and public health risks, and water monitoring should be considered for inclusion as a separate element in hazard analysis, as it often has a historical (accumulative) connotation.

    Item Type: Article
    Journal or Publication Title: Environ Res
    Additional Information: Lim, Yvonne A L Lai, Monica M Mahdy, Mohammed A K Mat Naim, H R Smith, Huw V eng Research Support, Non-U.S. Gov't 2009/08/12 09:00 Environ Res. 2009 Oct;109(7):857-9. Epub 2009 Aug 6.
    Uncontrolled Keywords: Animals Base Sequence DNA, Protozoan/chemistry/*genetics Fresh Water/*parasitology Giardia/*genetics/growth & development Humans Malaysia Microscopy, Interference Molecular Sequence Data Polymerase Chain Reaction Public Health Sequence Analysis, DNA Zoonoses/*parasitology
    Subjects: R Medicine
    Divisions: Faculty of Medicine > School of Medicine
    Depositing User: Ms Johana Johari
    Date Deposited: 11 Dec 2012 12:09
    Last Modified: 11 Dec 2012 12:09
    URI: http://eprints.um.edu.my/id/eprint/4112

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