Lim, Y.A.L.; Ramasame, S.D.; Mahdy, M.A.K.; Sulaiman, W.Y.W.; Smith, H.V. (2009) Detection and molecular characterization of Giardia isolated from recreational lake water in Malaysia. Parasitology Research, 106 (1). pp. 289-291. ISSN 1432-1955
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Abstract
Nine 50-l surface water samples from a Malaysian recreational lake were examined microscopically using an immunomagnetisable separation-immunofluorescent method. No Cryptosporidium oocysts were detected, but 77.8 of samples contained low numbers of Giardia cysts (range, 0.17-1.1 cysts/l), which were genetically characterised by SSU rRNA gene sequencing. Genotype analyses indicated the presence of Giardia duodenalis assemblage A suggesting potential risk to public health. The present study represents the first contribution to our knowledge of G. duodenalis assemblages in Malaysian recreational water.
| Item Type: | Article |
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| Creators: |
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| Journal or Publication Title: | Parasitology Research |
| Additional Information: | Lim, Yvonne A L Ramasame, Sharmila D Mahdy, Mohammed A K Sulaiman, Wan Yusoff W Smith, Huw V eng Germany 2009/08/26 09:00 Parasitol Res. 2009 Dec;106(1):289-91. |
| Uncontrolled Keywords: | Animals Cluster Analysis DNA, Protozoan/chemistry/genetics DNA, Ribosomal/chemistry/genetics Fresh Water/*parasitology Genes, rRNA Giardia/*classification/genetics/*isolation & purification Humans Immunomagnetic Separation/methods Malaysia Molecular Sequence Data Parasitology/methods Phylogeny RNA, Protozoan/genetics RNA, Ribosomal, 18S/genetics Sequence Analysis, DNA |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine > School of Medicine |
| Depositing User: | Ms Johana Johari |
| Date Deposited: | 11 Dec 2012 12:07 |
| Last Modified: | 11 Dec 2012 12:07 |
| URI: | http://eprints.um.edu.my/id/eprint/4110 |
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