Detection and molecular characterization of Giardia isolated from recreational lake water in Malaysia

Lim, Y.A.L.; Ramasame, S.D.; Mahdy, M.A.K.; Sulaiman, W.Y.W.; Smith, H.V. (2009) Detection and molecular characterization of Giardia isolated from recreational lake water in Malaysia. Parasitology Research, 106 (1). pp. 289-291. ISSN 1432-1955

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    Nine 50-l surface water samples from a Malaysian recreational lake were examined microscopically using an immunomagnetisable separation-immunofluorescent method. No Cryptosporidium oocysts were detected, but 77.8 of samples contained low numbers of Giardia cysts (range, 0.17-1.1 cysts/l), which were genetically characterised by SSU rRNA gene sequencing. Genotype analyses indicated the presence of Giardia duodenalis assemblage A suggesting potential risk to public health. The present study represents the first contribution to our knowledge of G. duodenalis assemblages in Malaysian recreational water.

    Item Type: Article
    1. Lim, Y.A.L.(Department of Parasitology, Faculty of Medicine University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    2. Ramasame, S.D.
    3. Mahdy, M.A.K.
    4. Sulaiman, W.Y.W.
    5. Smith, H.V.
    Journal or Publication Title: Parasitology Research
    Additional Information: Lim, Yvonne A L Ramasame, Sharmila D Mahdy, Mohammed A K Sulaiman, Wan Yusoff W Smith, Huw V eng Germany 2009/08/26 09:00 Parasitol Res. 2009 Dec;106(1):289-91.
    Uncontrolled Keywords: Animals Cluster Analysis DNA, Protozoan/chemistry/genetics DNA, Ribosomal/chemistry/genetics Fresh Water/*parasitology Genes, rRNA Giardia/*classification/genetics/*isolation & purification Humans Immunomagnetic Separation/methods Malaysia Molecular Sequence Data Parasitology/methods Phylogeny RNA, Protozoan/genetics RNA, Ribosomal, 18S/genetics Sequence Analysis, DNA
    Subjects: R Medicine
    Divisions: Faculty of Medicine > School of Medicine
    Depositing User: Ms Johana Johari
    Date Deposited: 11 Dec 2012 12:07
    Last Modified: 11 Dec 2012 12:07

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