Detection and molecular characterization of Giardia isolated from recreational lake water in Malaysia

Lim, Y.A.L.; Ramasame, S.D.; Mahdy, M.A.K.; Sulaiman, W.Y.W.; Smith, H.V. (2009) Detection and molecular characterization of Giardia isolated from recreational lake water in Malaysia. Parasitology Research, 106 (1). pp. 289-291. ISSN 1432-1955

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    Abstract

    Nine 50-l surface water samples from a Malaysian recreational lake were examined microscopically using an immunomagnetisable separation-immunofluorescent method. No Cryptosporidium oocysts were detected, but 77.8 of samples contained low numbers of Giardia cysts (range, 0.17-1.1 cysts/l), which were genetically characterised by SSU rRNA gene sequencing. Genotype analyses indicated the presence of Giardia duodenalis assemblage A suggesting potential risk to public health. The present study represents the first contribution to our knowledge of G. duodenalis assemblages in Malaysian recreational water.

    Item Type: Article
    Creators:
    1. Lim, Y.A.L.(Department of Parasitology, Faculty of Medicine University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    2. Ramasame, S.D.
    3. Mahdy, M.A.K.
    4. Sulaiman, W.Y.W.
    5. Smith, H.V.
    Journal or Publication Title: Parasitology Research
    Additional Information: Lim, Yvonne A L Ramasame, Sharmila D Mahdy, Mohammed A K Sulaiman, Wan Yusoff W Smith, Huw V eng Germany 2009/08/26 09:00 Parasitol Res. 2009 Dec;106(1):289-91.
    Uncontrolled Keywords: Animals Cluster Analysis DNA, Protozoan/chemistry/genetics DNA, Ribosomal/chemistry/genetics Fresh Water/*parasitology Genes, rRNA Giardia/*classification/genetics/*isolation & purification Humans Immunomagnetic Separation/methods Malaysia Molecular Sequence Data Parasitology/methods Phylogeny RNA, Protozoan/genetics RNA, Ribosomal, 18S/genetics Sequence Analysis, DNA
    Subjects: R Medicine
    Divisions: Faculty of Medicine > School of Medicine
    Depositing User: Ms Johana Johari
    Date Deposited: 11 Dec 2012 12:07
    Last Modified: 11 Dec 2012 12:07
    URI: http://eprints.um.edu.my/id/eprint/4110

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