Detection and molecular characterization of Giardia isolated from recreational lake water in Malaysia

Lim, Y.A.L. and Ramasame, S.D. and Mahdy, M.A.K. and Sulaiman, W.Y.W. and Smith, H.V. (2009) Detection and molecular characterization of Giardia isolated from recreational lake water in Malaysia. Parasitology Research, 106 (1). pp. 289-291. ISSN 1432-1955, DOI 19705155.

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Abstract

Nine 50-l surface water samples from a Malaysian recreational lake were examined microscopically using an immunomagnetisable separation-immunofluorescent method. No Cryptosporidium oocysts were detected, but 77.8 of samples contained low numbers of Giardia cysts (range, 0.17-1.1 cysts/l), which were genetically characterised by SSU rRNA gene sequencing. Genotype analyses indicated the presence of Giardia duodenalis assemblage A suggesting potential risk to public health. The present study represents the first contribution to our knowledge of G. duodenalis assemblages in Malaysian recreational water.

Item Type:	Article
Funders:	UNSPECIFIED
Additional Information:	Lim, Yvonne A L Ramasame, Sharmila D Mahdy, Mohammed A K Sulaiman, Wan Yusoff W Smith, Huw V eng Germany 2009/08/26 09:00 Parasitol Res. 2009 Dec;106(1):289-91.
Uncontrolled Keywords:	Animals Cluster Analysis DNA, Protozoan/chemistry/genetics DNA, Ribosomal/chemistry/genetics Fresh Water/*parasitology Genes, rRNA Giardia/*classification/genetics/*isolation & purification Humans Immunomagnetic Separation/methods Malaysia Molecular Sequence Data Parasitology/methods Phylogeny RNA, Protozoan/genetics RNA, Ribosomal, 18S/genetics Sequence Analysis, DNA
Subjects:	R Medicine
Divisions:	Faculty of Medicine > School of Medicine
Depositing User:	Ms Johana Johari
Date Deposited:	11 Dec 2012 04:07
Last Modified:	11 Dec 2012 04:07
URI:	http://eprints.um.edu.my/id/eprint/4110

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