Fong, M.Y.; Lau, Y.L.; Init, I.; Jamaiah, I.; Anuar, A.K.; Rahmah, N. (2003) Recombinant expression of Toxocara canis excretory-secretory antigen TES-120 in Escherichia coli. The Southeast Asian Journal of Tropical Medicine and Public Health, 34 (4). pp. 723-726. ISSN 0125-1562
The gene encoding the excretory-secretory antigen TES-120 of dog ascarid worm Toxocara canis was cloned into the bacterium Escherichia coli. The specificity of the recombinant TES-120 antigen produced by the bacterium was investigated. A total of 45 human serum samples from patients infected with differenthelminthes and protozoa, including 8 cases of toxocariasis, were tested against the recombinant antigens in immunoblot assays. The results from the assays revealed that the recombinant TES-120 antigen reacted with sera from toxocariasis patients only. This highly specific recombinant TES-120 antigen can potentially be used for the development of an inexpensive serodiagnostic assay for human toxocariasis.
|Item Type: ||Article|
- Fong, M.Y.
- Lau, Y.L.
- Init, I.( Department of Parasitology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
- Jamaiah, I.
- Anuar, A.K.
- Rahmah, N.
|Journal or Publication Title: ||The Southeast Asian Journal of Tropical Medicine and Public Health|
|Additional Information: ||Fong, Mun-Yik Lau, Yee-Ling Init, Ithoi Jamaiah, I Anuar, A Khairul Rahmah, N eng Evaluation Studies Research Support, Non-U.S. Gov't Thailand 2004/04/30 05:00 Southeast Asian J Trop Med Public Health. 2003 Dec;34(4):723-6.|
|Uncontrolled Keywords: ||Antigens, Helminth/*diagnostic use/metabolism Cloning, Molecular Dogs Escherichia coli/metabolism Helminth Proteins/*diagnostic use/metabolism Humans Immunoblotting Larva Migrans/*diagnosis/immunology Recombinant Proteins/*diagnostic use/metabolism Sensitivity and Specificity Toxocara canis/*immunology|
|Subjects: ||R Medicine|
|Divisions: ||Faculty of Medicine|
|Depositing User: ||Mr Zainal Sabri|
|Date Deposited: ||10 Dec 2012 09:53|
|Last Modified: ||10 Dec 2012 09:53|
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