Recombinant expression of Toxocara canis excretory-secretory antigen TES-120 in Escherichia coli

Fong, M.Y.; Lau, Y.L.; Init, I.; Jamaiah, I.; Anuar, A.K.; Rahmah, N. (2003) Recombinant expression of Toxocara canis excretory-secretory antigen TES-120 in Escherichia coli. The Southeast Asian Journal of Tropical Medicine and Public Health, 34 (4). pp. 723-726. ISSN 0125-1562

[img]
Preview
PDF - Published Version [error in script]
Download (50Kb) | Preview

    Abstract

    The gene encoding the excretory-secretory antigen TES-120 of dog ascarid worm Toxocara canis was cloned into the bacterium Escherichia coli. The specificity of the recombinant TES-120 antigen produced by the bacterium was investigated. A total of 45 human serum samples from patients infected with differenthelminthes and protozoa, including 8 cases of toxocariasis, were tested against the recombinant antigens in immunoblot assays. The results from the assays revealed that the recombinant TES-120 antigen reacted with sera from toxocariasis patients only. This highly specific recombinant TES-120 antigen can potentially be used for the development of an inexpensive serodiagnostic assay for human toxocariasis.

    Item Type: Article
    Creators:
    1. Fong, M.Y.
    2. Lau, Y.L.
    3. Init, I.( Department of Parasitology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    4. Jamaiah, I.
    5. Anuar, A.K.
    6. Rahmah, N.
    Journal or Publication Title: The Southeast Asian Journal of Tropical Medicine and Public Health
    Additional Information: Fong, Mun-Yik Lau, Yee-Ling Init, Ithoi Jamaiah, I Anuar, A Khairul Rahmah, N eng Evaluation Studies Research Support, Non-U.S. Gov't Thailand 2004/04/30 05:00 Southeast Asian J Trop Med Public Health. 2003 Dec;34(4):723-6.
    Uncontrolled Keywords: Antigens, Helminth/*diagnostic use/metabolism Cloning, Molecular Dogs Escherichia coli/metabolism Helminth Proteins/*diagnostic use/metabolism Humans Immunoblotting Larva Migrans/*diagnosis/immunology Recombinant Proteins/*diagnostic use/metabolism Sensitivity and Specificity Toxocara canis/*immunology
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Mr Jenal S
    Date Deposited: 10 Dec 2012 09:53
    Last Modified: 10 Dec 2012 09:53
    URI: http://eprints.um.edu.my/id/eprint/4109

    Actions (For repository staff only: Login required)

    View Item

    Document Downloads

    More statistics for this item...