Lum, Wai Mun and Lim, Hong Chang and Lau, Winnie Lik Sing and Law, Ing Kuo and Teng, Sing Tung and Benico, Garry and Leong, Sandric Chee Yew and Takahashi, Kazuya and Gu, Haifeng and Lirdwitayaprasit, Thaithaworn and Leaw, Chui Pin and Lim, Po Teen and Iwataki, Mitsunori (2022) Description of two new species Chattonella tenuiplastida sp. nov. and Chattonella malayana sp. nov. (Raphidophyceae) from South China Sea, with a report of wild fish mortality. Harmful Algae, 118. ISSN 1568-9883, DOI https://doi.org/10.1016/j.hal.2022.102322.
Full text not available from this repository.Abstract
Fisheries damage caused by Chattonella red tide has been recorded in Southeast Asia. Molecular studies have clarified the presence of two species, Chattonella marina complex and Chattonella subsalsa in the region, unlike East Asia that had only C. marina complex. To elucidate the phylogeography of Chattonella in Asia, further phylogenetic and morphological examinations were carried out with 33 additional culture strains, including the strains isolated during a bloom of Chattonella sp. (up to 142 cells mL(-1)) that was associated with a wild fish mortality along the northeastern coast of Peninsular Malaysia in 2016, and those from Yellow Sea, where the Chattonella genotypes have not been determined. LSU rDNA and ITS2 trees showed five intrageneric clades in the genus Chattonella, which were clades I and II (C. subsalsa), Glade III (C. marina complex) and two new clades, namely Glade IV from Thailand and Malaysia, and Glade V from Peninsular Malaysia. The positions of the two new clades were different in LSU rDNA and ITS2 trees. LSU rDNA divergences of clades IV and V from the other clades were >= 4.01% and >= 5.70%, while their ITS2 divergences were >= 7.44% and >= 16.43%, respectively. Three and five compensatory base changes (CBCs) were observed in the clades IV and V, respectively, when compared to each of their closest Glade. Cells from clades IV and V showed similar morphology to C. marina complex and C. subsalsa Glade II, including the presence of button-like granules on cell surface and oboe-shaped mucocysts. However, cell size, the number and shape of chloroplasts in Chattonella clades IV and V, and the nonstacked thylakoids penetrated the pyrenoid in C. subsalsa Glade II, were distinctive. Based on the diagnostic chloroplast shape, we proposed the designation of clades IV and V to two new species, Chattonella tenuiplastida sp. nov. and Chattonella malayana sp. nov.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Education, Malaysia (FRGS/1/2017/STG03/TARUC/02/1), Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT) Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research (KAKENHI) (19H03027), Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT) Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research (KAKENHI) (19KK0160), Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT), Ministry of Higher Education MyBrain Scholarship |
| Uncontrolled Keywords: | Fish kill; Chattonella malayana sp. nov.; Chattonella tenuiplastida sp. nov.; ITS2; LSU rDNA; South China Sea; Yellow Sea |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Divisions: | Deputy Vice Chancellor (Research & Innovation) Office > Institute of Ocean and Earth Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 17 Aug 2023 07:04 |
| Last Modified: | 17 Aug 2023 07:04 |
| URI: | http://eprints.um.edu.my/id/eprint/41067 |
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