Molecular characterization of Leishmania species isolated from cutaneous leishmaniasis in Yemen

Mahdy, M.A.; Al-Mekhlafi, H.M.; Al-Mekhlafi, A.M.; Lim, Y.A.; Bin Shuaib, N.O.; Azazy, A.A.; Mahmud, R. (2010) Molecular characterization of Leishmania species isolated from cutaneous leishmaniasis in Yemen. PLoS One, 5 (9). ISSN 1932-6203

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    Abstract

    Background Cutaneous leishmaniasis (CL) is a neglected tropical disease endemic in the tropics and subtropics with a global yearly incidence of 1.5 million. Although CL is the most common form of leishmaniasis, which is responsible for 60 of DALYs lost due to tropical-cluster diseases prevalent in Yemen, available information is very limited. Methodology/Principal Findings This study was conducted to determine the molecular characterization of Leishmania species isolated from human cutaneous lesions in Yemen. Dermal scrapes were collected and examined for Leishmania amastigotes using the Giemsa staining technique. Amplification of the ribosomal internal transcribed spacer 1(ITS-1) gene was carried out using nested PCR and subsequent sequencing. The sequences from Leishmania isolates were subjected to phylogenetic analysis using the neighbor-joining and maximum parsimony methods. The trees identified Leishmania tropica from 16 isolates which were represented by two sequence types. The predominance of the anthroponotic species (i.e. L. tropica) indicates the probability of anthroponotic transmission of cutaneous leishmaniasis in Yemen. These findings will help public health authorities to build an effective control strategy taking into consideration person�to-person transmission as the main dynamic of transmission of CL.

    Item Type: Article
    Creators:
    1. Mahdy, M.A.
    2. Al-Mekhlafi, H.M.
    3. Al-Mekhlafi, A.M.
    4. Lim, Y.A.(Department of Parasitology, Faculty of Medicine University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    5. Bin Shuaib, N.O.
    6. Azazy, A.A.
    7. Mahmud, R.
    Journal or Publication Title: PLoS One
    Additional Information: Mahdy, Mohammed A K Al-Mekhlafi, Hesham M Al-Mekhlafi, Abdulsalam M Lim, Yvonne A L Bin Shuaib, Naemah O M Azazy, Ahmed A Mahmud, Rohela eng 2010/09/24 06:00 PLoS One. 2010 Sep 20;5(9). pii: e12879.
    Uncontrolled Keywords: Humans Leishmania/classification/*genetics/*isolation & purification Leishmaniasis, Cutaneous/epidemiology/*parasitology Molecular Sequence Data Phylogeny Yemen/epidemiology
    Subjects: R Medicine
    Divisions: Faculty of Medicine > School of Medicine
    Depositing User: Ms Johana Johari
    Date Deposited: 10 Dec 2012 09:34
    Last Modified: 10 Dec 2012 09:34
    URI: http://eprints.um.edu.my/id/eprint/4095

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