Morphological characteristics of developmental stages of acanthamoeba and naegleria species before and after staining by various techniques

Ithoi, I.; Ahmad, A.F.; Mak, J.W.; Nissapatorn, V.; Lau, Y.L.; Mahmud, R. (2011) Morphological characteristics of developmental stages of acanthamoeba and naegleria species before and after staining by various techniques. Southeast Asian Journal of Tropical Medicine and Public Health, 42 (6). pp. 1327-1338. ISSN 0125-1562

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    Abstract

    Seven stains were studied to determine the best color and contrast for staining the developmental stages of free living pathogenic Acanthamoeba and Naegleria species. The acid-fast bacilli stain (AFB) produced a blue color without contrast; trichrome-eosin and modified Field's showed various color contrasts; Giemsa, iron-hematoxylin, modified AFB and Gram produced only one color which distinguished the nucleus, nucleolus, cytoplasm, food- and water-vacuoles. The motile organs (acanthopodia, pseudopodia, lobopodia and flagella) were also clearly differentiated but produced a similar color as the cytoplasm. These motile organelles were first induced by incubating at 37 C for at least 15 minutes and then fixing with methanol in order to preserve the protruding morphology prior to staining. The trichrome-eosin and iron-hematoxylin stains showed good color contrast for detecting all three stages, the trophozoite, cyst and flagellate; Giemsa and Gram stained the trophozoite and flagellate stages; the modified Field's and modified AFB stains stained only the trophozoite stage. Depending on the purpose, all these stains (except the AFB stain) can be used to identify the developmental stages of Acanthanweba and Naegleria for clinical, epidemiological or public health use.

    Item Type: Article
    Journal or Publication Title: Southeast Asian Journal of Tropical Medicine and Public Health
    Additional Information: Ithoi, Init Ahmad, Arine-Fadzlun Mak, J. W. Nissapatorn, Veeranoot Lau, Yee-Ling Mahmud, Rohela
    Uncontrolled Keywords: Acanthamoeba naegleria stains developmental stages keratitis disease agents
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Mr Jenal S
    Date Deposited: 06 Dec 2012 16:27
    Last Modified: 06 Dec 2012 16:27
    URI: http://eprints.um.edu.my/id/eprint/4085

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