Semail, Nadhiratul-Farihin and Keyon, Aemi Syazwani Abdul and Saad, Bahruddin and Kamaruzaman, Sazlinda and Zain, Nur Nadhirah Mohamad and Lim, Vuanghao and Miskam, Mazidatulakmam and Abdullah, Wan Nazwanie Wan and Ramachandran, Muggundha Raoov and Yahaya, Noorfatimah (2022) Induced sample via transient isotachophoresis mediated with sweeping in micellar electrokinetic chromatography for the dual-stacking strategy of non-steroidal anti-inflammatory drugs in environmental water samples. Journal of Chromatography A, 1685. ISSN 0021-9673, DOI https://doi.org/10.1016/j.chroma.2022.463616.
Full text not available from this repository.Abstract
Realising the need to devise a simple, sensitive, and reliable detection method, this study investigated the development of a dual-stacking transient isotachophoresis (t-ITP) and sweeping in micellar electrokinetic chromatography with diode array detector (t-ITP/sweeping-MEKC-DAD) for the determination of selected non-steroidal anti-inflammatory drugs (NSAIDs); ketoprofen, diclofenac and naproxen from aqueous ma-trices. Prior to the system setup, various parameters were optimised to assess the potential use of the t-ITP paired with the sweeping stacking technique in micellar background electrolyte for dual preconcen-tration and separation of trace amounts of NSAIDs. Once the optimum conditions have been established, the method performance was validated and applied to 17 environmental water samples. Based on the results, the combined t-ITP and sweeping approach significantly improved the stacking and separation sensitivity. A large volume of samples could also be introduced and subsequently separated by MEKC with greater focusing effects due to the sweeping. Under optimised conditions, the developed method exhibited excellent linearity at a high range (0.1-500 ng/mL, r 2 >= 0.998), low limits of detection (LODs) of 0.01-0.07 ng/mL, and a remarkable relative recovery (RR) of 99.6-101.9% with a relative standard devi-ation (RSD) of 1.4-8.6% ( n = 9). Ultimately, the sensitivity enhancement factors improved up to 666-fold using the optimised method. Therefore, the proposed method presents a simplified yet effective and suit-able for the determination of NSAIDs from aqueous matrices.(c) 2022 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Higher Education Malaysia through Fundamental Research Grant Scheme (FRGS) (Grant No: FRGS/1/2021/STG04/USM/02/4 & Code 203.CIPPT.6711973) |
| Uncontrolled Keywords: | Transient isotachophoresis; Sweeping; Micellar electrokinetic chromatography; Non-steroidal anti-inflammatory drugs; Environmental sample |
| Subjects: | Q Science > QD Chemistry |
| Divisions: | Faculty of Science > Department of Chemistry |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 14 Nov 2023 03:31 |
| Last Modified: | 14 Nov 2023 03:31 |
| URI: | http://eprints.um.edu.my/id/eprint/40724 |
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