Detection of immunogenic parasite and host-specific proteins in the sera of active and chronic individuals infected with Toxoplasma gondii
Yeng, C.; Osman, E.; Mohamed, Z.; Noordin, R. (2010) Detection of immunogenic parasite and host-specific proteins in the sera of active and chronic individuals infected with Toxoplasma gondii. Electrophoresis, 31 (23-24). pp. 3843-3849. ISSN 0173-0835
Toxoplasma gondii infection in pregnant women may result in abortion and foetal abnormalities, and may be life-threatening in immunocompromised hosts. To identify the potential infection markers of this disease, 2-DE and Western blot methods were employed to study the parasite circulating antigens and host-specific proteins in the sera of T. gondii-infected individuals. The comparisons were made between serum protein profiles of infected (n = 31) and normal (n = 10) subjects. Antigenic proteins were identified by immunoblotting using pooled sera and monoclonal anti-human IgM-HRP. Selected protein spots were characterised using mass spectrometry. Prominent differences were observed when serum samples of T. gondii-infected individuals and normal controls were compared. A significant up-regulation of host-specific proteins, alpha(2)-HS glycoprotein and alpha(1)-B glycoprotein, was also observed in the silver-stained gels of both active and chronic infections. However, only alpha(2)-HS glycoprotein and alpha(1)-B glycoprotein in the active infection showed immunoreactivity in Western blots. In addition, three spots of T. gondii proteins were detected, namely (i) hypothetical protein chrXII: 3984434-3 TGME 49, (ii) dual specificity protein phosphatase, catalytic domain TGME 49 and (iii) NADPH-cytochrome p450 reductase TGME 49. Thus, 2-DE approach followed by Western blotting has enabled the identification of five potential infection markers for the diagnosis of toxoplasmosis: three are parasite-specific proteins and two are host-specific proteins.
|Item Type: ||Article|
- Yeng, C.
- Osman, E.
- Mohamed, Z.
- Noordin, R.
|Journal or Publication Title: ||Electrophoresis|
|Additional Information: ||ISI Document Delivery No.: 695UO Times Cited: 0 Cited Reference Count: 34 Cited References: ARAUJO FG, 1980, INFECT IMMUN, V30, P12 Attallah AM, 2006, J IMMUNOASS IMMUNOCH, V27, P45, DOI 10.1080/15321810500403748 Chen Y, 2008, PROTEOME SCI, V6, DOI 10.1186/1477-5956-6-20 Dubey JP, 1988, TOXOPLASMOSIS ANIMAL Dybas JM, 2008, PLOS ONE, V3, DOI 10.1371/journal.pone.0003899 Emelia O, 2010, J IMMUNOASS IMMUNOCH, V31, P79, DOI 10.1080/15321810903405134 Gangadharan B, 2007, CLIN CHEM, V53, P1792, DOI 10.1373/clinchem.2007.089144 Gastens MH, 2002, INT J PARASITOL, V32, P1225, DOI 10.1016/S0020-7519(02)00096-6 GIRMA JP, 1987, BLOOD, V70, P605 GLADKOVA SV, 2000, PARASITOLOGY, V4, P15 GROSS U, 1993, PARASITOL RES, V79, P191, DOI 10.1007/BF00931891 Guigo Roderic, 2006, Genome Biol, V7 Suppl 1 He QY, 2003, PROTEOMICS, V3, P666, DOI 10.1002/pmic.200300394 He QY, 2008, DIGEST DIS SCI, V53, P3112, DOI 10.1007/s10620-008-0269-z HEUKESHOVEN J, 1988, ELECTROPHORESIS, V9, P28, DOI 10.1002/elps.1150090106 Huynh MH, 2003, EMBO J, V22, P2082, DOI 10.1093/emboj/cdg217 Jethwaney D, 2005, INFECT IMMUN, V73, P5883, DOI 10.1128/IAI.73.9.5883-5891.2005 Jones JL, 2001, AM J EPIDEMIOL, V154, P357, DOI 10.1093/aje/154.4.357 Lee IN, 2006, PROTEOMICS, V6, P2865, DOI 10.1002/pmic.200500488 Lu FL, 2007, BMC GENOMICS, V8, DOI 10.1186/1471-2164-8-255 Ma GY, 2009, EXP PARASITOL, V122, P41, DOI 10.1016/j.exppara.2009.01.011 MARIA G, 1998, REV SOC BRAS MED TRO, V31, P271 Monteiro VG, 1998, FEMS MICROBIOL LETT, V164, P323, DOI 10.1111/j.1574-6968.1998.tb13105.x Neudeck A, 2002, MICROBES INFECT, V4, P581, DOI 10.1016/S1286-4579(02)01576-9 Nielsen HV, 2005, J CLIN MICROBIOL, V43, P711, DOI 10.1128/JCM.43.2.711-715.2005 Nielsen P, 2005, BIOINFORMATICS, V21, P4322, DOI 10.1093/bioinformatics/bti701 Nischik N, 2001, MICROBES INFECT, V3, P689, DOI 10.1016/S1286-4579(01)01425-3 Reichmann G, 2001, MICROBES INFECT, V3, P779, DOI 10.1016/S1286-4579(01)01434-4 Shevchenko A, 1996, ANAL CHEM, V68, P850, DOI 10.1021/ac950914h Shojaee S, 2007, PAK J MED SCI, V23, P100 Susanto L, 2001, Southeast Asian J Trop Med Public Health, V32 Suppl 2, P195 T, 2000, INT J PARASITOL, V30, P1217 Thayan R, 2009, T ROY SOC TROP MED H, V103, P413, DOI 10.1016/j.trstmh.2008.12.018 Vonlaufen N, 2007, PARASITOLOGY, V134, P805, DOI 10.1017/S0031182006002186 Yeng, Chen Osman, Emelia Mohamed, Zeehaida Noordin, Rahmah MOSTI02-01-05-SF0154, 305/CIPPM/613206; USM Research University1001/CIPPM/8130132 We thank Associate Professor Dr. Gam Lay Harn for technical advice rendered in the initial phase of the study. This research is supported by research grants from MOSTI Science Fund No. 02-01-05-SF0154 (USM No. 305/CIPPM/613206) and USM Research University (RU) fund (No. 1001/CIPPM/8130132). Wiley-blackwell Malden|
|Uncontrolled Keywords: ||2-DE Host-specific protein Immunoblot Parasite protein Toxoplasma gondii adhesive protein hepatitis-c identification antigens fetuin inflammation expression proteomics diagnosis biomarker|
|Subjects: ||R Medicine > RK Dentistry|
|Divisions: ||Faculty of Dentistry|
|Depositing User: ||Ms Nursyafiqah Abd Malek|
|Date Deposited: ||06 Dec 2012 14:54|
|Last Modified: ||06 Dec 2012 14:54|
Actions (login required)