Detection of immunogenic parasite and host-specific proteins in the sera of active and chronic individuals infected with Toxoplasma gondii

Yeng, C. and Osman, E. and Mohamed, Z. and Noordin, R. (2010) Detection of immunogenic parasite and host-specific proteins in the sera of active and chronic individuals infected with Toxoplasma gondii. Electrophoresis, 31 (23-24). pp. 3843-3849. ISSN 0173-0835, DOI https://doi.org/10.1002/elps.201000038.

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Abstract

Toxoplasma gondii infection in pregnant women may result in abortion and foetal abnormalities, and may be life-threatening in immunocompromised hosts. To identify the potential infection markers of this disease, 2-DE and Western blot methods were employed to study the parasite circulating antigens and host-specific proteins in the sera of T. gondii-infected individuals. The comparisons were made between serum protein profiles of infected (n = 31) and normal (n = 10) subjects. Antigenic proteins were identified by immunoblotting using pooled sera and monoclonal anti-human IgM-HRP. Selected protein spots were characterised using mass spectrometry. Prominent differences were observed when serum samples of T. gondii-infected individuals and normal controls were compared. A significant up-regulation of host-specific proteins, alpha(2)-HS glycoprotein and alpha(1)-B glycoprotein, was also observed in the silver-stained gels of both active and chronic infections. However, only alpha(2)-HS glycoprotein and alpha(1)-B glycoprotein in the active infection showed immunoreactivity in Western blots. In addition, three spots of T. gondii proteins were detected, namely (i) hypothetical protein chrXII: 3984434-3 TGME 49, (ii) dual specificity protein phosphatase, catalytic domain TGME 49 and (iii) NADPH-cytochrome p450 reductase TGME 49. Thus, 2-DE approach followed by Western blotting has enabled the identification of five potential infection markers for the diagnosis of toxoplasmosis: three are parasite-specific proteins and two are host-specific proteins.

Item Type: Article
Funders: UNSPECIFIED
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Uncontrolled Keywords: 2-DE Host-specific protein Immunoblot Parasite protein Toxoplasma gondii adhesive protein hepatitis-c identification antigens fetuin inflammation expression proteomics diagnosis biomarker
Subjects: R Medicine > RK Dentistry
Divisions: Faculty of Dentistry
Depositing User: Ms Nursyafiqah Abd Malek
Date Deposited: 06 Dec 2012 06:54
Last Modified: 06 Dec 2012 06:54
URI: http://eprints.um.edu.my/id/eprint/4055

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