First molecular characterization of Giardia duodenalis from goats in Malaysia

Lim, Y.A.L.; Mahdy, M.A.K.; Tan, T.K.; Goh, X.T.; Jex, A.R.; Nolan, M.J.; Sharma, R.S.K.; Gasser, R.B. (2012) First molecular characterization of Giardia duodenalis from goats in Malaysia. Molecular and Cellular Probes. pp. 1-4. ISSN 0890-8508

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    Abstract

    In the present study, 310 faecal samples from goats from eight different farms in Malaysia were tested for the presence of Giardia using a PCR-coupled approach. The nested PCR for SSU amplified products of the expected size (�200 bp) from 21 of 310 (6.8) samples. Sixteen of these 21 products could be sequenced successfully and represented six distinct sequence types. Phylogenetic analysis of the SSU sequence data using Bayesian Inference (BI) identified Giardia assemblages A, B and E. The identification of the 'zoonotic' assemblages A and B suggests that Giardia-infected goats represent a possible reservoir for human giardiasis in Malaysia.

    Item Type: Article
    Creators:
    1. Lim, Y.A.L.(Department of Parasitology, Faculty of Medicine University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    2. Mahdy, M.A.K.
    3. Tan, T.K.
    4. Goh, X.T.
    5. Jex, A.R.
    6. Nolan, M.J.
    7. Sharma, R.S.K.
    8. Gasser, R.B.
    Journal or Publication Title: Molecular and Cellular Probes
    Additional Information: Department of Parasitology, Faculty of Medicine University of Malaya, 50603 Kuala Lumpur, MALAYSIA
    Uncontrolled Keywords: Giardia duodenalis Small subunit of the nuclear ribosomal RNA (SSU) gene Goats Malaysia
    Subjects: R Medicine
    Divisions: Faculty of Medicine > School of Medicine
    Depositing User: Ms Johana Johari
    Date Deposited: 06 Dec 2012 16:24
    Last Modified: 06 Dec 2012 16:24
    URI: http://eprints.um.edu.my/id/eprint/4054

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