Molecular identification of human hookworm infections in economically disadvantaged communities in peninsular Malaysia

Ngui, R.; Ching, L.S.; Kai, T.T.; Roslan, M.A.; Lim, Y.A.L. (2012) Molecular identification of human hookworm infections in economically disadvantaged communities in peninsular Malaysia. American Journal of Tropical Medicine and Hygiene, 86 (5). pp. 837-842. ISSN 0002-9637

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Abstract

Species identification of human hookworm infections among eight communities in rural areas of Peninsular Malaysia was determined during 2009-2011. Fecal samples were examined by microscopy and subsequently, the internal transcribed spacer 2 and 28S ribosomal RNA region of Necator americanus and Ancylostoma spp. were sequenced. Overall, 9.1 (58 of 634) were identified positive by microscopy for hookworm infection, and 47 (81.0) of 58 were successfully amplified and sequenced. Sequence comparison found that N. americanus (87.2) was the most predominant hookworm identified, followed by Ancylostoma ceylanicum (23.4). No A. duodenale infection was detected in this study. Detection of A. ceylanicum in humans highlighted the zoonotic transmission among humans living near dogs. Thus, implementation of effective control measures for hookworm infections in future should seriously consider this zoonotic implication.

Item Type: Article
Creators:
  1. Ngui, R.
  2. Ching, L.S.
  3. Kai, T.T.
  4. Roslan, M.A.
  5. Lim, Y.A.L.(Department of Parasitology, Faculty of Medicine University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
Journal or Publication Title: American Journal of Tropical Medicine and Hygiene
Additional Information: Ngui, Romano Ching, Lee Soo Kai, Tan Tiong Roslan, Muhammad Aidil Lim, Yvonne A. L.
Uncontrolled Keywords: Adolescent Adult Aged Aged, 80 and over Ancylostoma/*genetics/isolation & purification/pathogenicity Animals Child Child, Preschool DNA, Helminth/*genetics/isolation & purification DNA, Ribosomal Spacer/genetics Dogs Feces/parasitology Female Hookworm Infections/*diagnosis/*epidemiology/*parasitology/veterinary Host-Pathogen Interactions Humans Malaysia/epidemiology Male Middle Aged Necator americanus/*genetics/isolation & purification/pathogenicity RNA, Ribosomal, 28S/genetics Sequence Analysis, DNA Sequence Analysis, RNA Socioeconomic Factors Young Adult
Subjects: R Medicine
Divisions: Faculty of Medicine > School of Medicine
Depositing User: Ms Johana Johari
Date Deposited: 05 Dec 2012 11:53
Last Modified: 05 Dec 2012 11:53
URI: http://eprints.um.edu.my/id/eprint/4049

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