Extraction, isolation and characterization of bioactive compounds from plants' extracts

Sasidharan, S.; Chen, Y.; Saravanan, D.; Sundram, K.M.; Latha, L.Y. (2011) Extraction, isolation and characterization of bioactive compounds from plants' extracts. African Journal of Traditional Complementary and Alternative Medicines, 8 (1). pp. 1-10. ISSN 0189-6016

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    Natural products from medicinal plants, either as pure compounds or as standardized extracts, provide unlimited opportunities for new drug leads because of the unmatched availability of chemical diversity. Due to an increasing demand for chemical diversity in screening programs, seeking therapeutic drugs from natural products, interest particularly in edible plants has grown throughout the world. Botanicals and herbal preparations for medicinal usage contain various types of bioactive compounds. The focus of this paper is on the analytical methodologies, which include the extraction, isolation and characterization of active ingredients in botanicals and herbal preparations. The common problems and key challenges in the extraction, isolation and characterization of active ingredients in botanicals and herbal preparations are discussed. As extraction is the most important step in the analysis of constituents present in botanicals and herbal preparations, the strengths and weaknesses of different extraction techniques are discussed. The analysis of bioactive compounds present in the plant extracts involving the applications of common phytochemical screening assays, chromatographic techniques such as HPLC and, TLC as well as non-chromatographic techniques such as immunoassay and Fourier Transform Infra Red (FTIR) are discussed.

    Item Type: Article
    1. Sasidharan, S.
    2. Chen, Y.
    3. Saravanan, D.
    4. Sundram, K.M.
    5. Latha, L.Y.
    Journal or Publication Title: African Journal of Traditional Complementary and Alternative Medicines
    Additional Information: ISI Document Delivery No.: 661UY Times Cited: 0 Cited Reference Count: 38 Cited References: Akinyemi Kabir O, 2005, BMC Complement Altern Med, V5, P6, DOI 10.1186/1472-6882-5-6 Cai ZW, 2002, J MASS SPECTROM, V37, P1013, DOI 10.1002/jms.370 CANNELL RJP, 1998, NATURAL PRODUCTS ISO, P165 CHANDA SV, 2006, AFR J BIOMED RES, V9, P53 COSA P, 2006, J ETHNOPHARMACOL, V106, P290 Cunha IBS, 2004, J BRAZIL CHEM SOC, V15, P964, DOI 10.1590/S0103-50532004000600026 Dahiru D., 2006, African Journal of Traditional, Complementary and Alternative Medicines, V3, P70 Duraipandiyan Veeramuthu, 2006, BMC Complement Altern Med, V6, P35, DOI 10.1186/1472-6882-6-35 Eberhardt TL, 2007, WOOD FIBER SCI, V39, P319 Edeoga HO, 2005, AFR J BIOTECHNOL, V4, P685 Fabricant DS, 2001, ENVIRON HEALTH PERSP, V109, P69, DOI 10.2307/3434847 Fan XH, 2006, ANAL CHIM ACTA, V555, P217, DOI 10.1016/j.aca.2005.09.037 HAMBURGER MO, 1987, J NAT PROD, V50, P19, DOI 10.1021/np50049a003 Hazra KM, 2007, AFR J BIOTECHNOL, V6, P1446 He XG, 2000, J CHROMATOGR A, V880, P203, DOI 10.1016/S0021-9673(00)00059-5 HOLSTEGE DM, 1995, J AGR FOOD CHEM, V43, P691, DOI 10.1021/jf00051a025 HOMANS AL, 1970, J CHROMATOGR, V51, P327, DOI 10.1016/S0021-9673(01)96877-3 Huie CW, 2002, ANAL BIOANAL CHEM, V373, P23, DOI 10.1007/s00216-002-1265-3 Kumar GS, 2007, TROP J PHARM RES, V6, P717 Li HB, 2004, J CHROMATOGR B, V812, P277, DOI 10.1016/j.jchromb.2004.06.045 Mallikharjuna P. B., 2007, E-Journal of Chemistry, V4, P510 MATTOCKS AR, 1967, ANAL CHEM, V39, P443, DOI 10.1021/ac60248a006 McGaw LJ, 2007, J ETHNOPHARMACOL, V110, P16, DOI 10.1016/j.jep.2006.08.029 Onwukaeme DN, 2007, TROP J PHARM RES, V6, P725 Parekh Jigna, 2007, Turkish Journal of Biology, V31, P53 Phrompittayarat W, 2007, NARESUAN U J, V15, P29 RAHALISON L, 1991, PHYTOCHEM ANALYSIS, V2, P199, DOI 10.1002/pca.2800020503 Sasidharan S, 2008, PHARM BIOL, V46, P413, DOI 10.1080/13880200802055867 Shahverdi AR, 2007, J CHROMATOGR B, V850, P528, DOI 10.1016/j.jchromb.2006.11.011 SHOYAMA Y, 2003, CYTOTECHNOLOGY, V31, P9 The Pharmacopoeia Commission of PRC, 2000, PHARM PEOPL REP CHIN The Society of Japanese Pharmacopoeia, 2001, JAP PHARM Tona L, 1998, J ETHNOPHARMACOL, V61, P57, DOI 10.1016/S0378-8741(98)00015-4 Tsao R, 2004, J CHROMATOGR B, V812, P85, DOI 10.1016/j.jchromb.2004.09.028 Woisky RG, 1998, J APICULT RES, V37, P99 Ye M, 2007, J AM SOC MASS SPECTR, V18, P82, DOI 10.1016/j.jasms.2006.08.009 Zygmunt B, 2003, J CHROMATOGR SCI, V41, P109 *US PHARM CONV INC, 2002, US PHARM NAT FORM Sasidharan, S. Chen, Y. Saravanan, D. Sundram, K. M. Latha, L. Yoga African networks ethnomedicines Ile-ife
    Uncontrolled Keywords: Bioactive compound Plant Extraction Isolation Herbal preparations Natural products indian medicinal-plants phytochemical constituents pyrrolizidine alkaloids mass-spectrometry quality-control propolis samples assay
    Subjects: R Medicine > RK Dentistry
    Divisions: Faculty of Dentistry
    Depositing User: Ms Nursyafiqah Abd Malek
    Date Deposited: 05 Dec 2012 12:07
    Last Modified: 05 Dec 2012 12:07
    URI: http://eprints.um.edu.my/id/eprint/4045

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