Geng, Huijuan and Gopinath, Subash Cb and Chen, Yeng and Niu, Wenyan (2022) Sensitive aptasensing of hepatitis B viral antigen gold dielectrode by current-volt measurements. Journal of Nanoelectronics and Optoelectronics, 17 (7). pp. 1011-1018. ISSN 1555-1318, DOI https://doi.org/10.1166/jno.2022.3288.
Full text not available from this repository.Abstract
Hepatitis B virus (HBV) is a serious human health issue, primarily leading to cirrhosis, chronic hepatitis, and primary liver cancer. Patients are at higher risk of a poor prognosis if the viral load exceeds >10(5) copies/mL; hence, it is crucial to identify the early stages of HBV infection. This research aimed to identify hepatitis B surface antigen (HBsAg) on an interdigitated gold electrode sensor assisted by HBsAg-specific aptamer and antibody interactions. The aptamer-HBsAg-antibody sandwich assay was carried out on an interdigitated electrode using aptamer as the capture molecule and anti-HBsAg antibody as the detection molecule. Higher capture probe immobilization was achieved through the gold nanourchin conjugated capture aptamer. On the aptamer-attached electrode, HBsAg interacted with and was sandwiched by the detection antibody. The sandwich assay lowered the limit of HBsAg detection to 0.1 ng/mL, as indicated by the calculated R-2 value of 0.9915 on a linear regression graph presenting data for concentrations of HBsAg from 0.1 to 60 ng/mL. Furthermore, specific HBsAg detection was confirmed by complementarily of the capture aptamer sequence and nonspecific proteins (hepatitis C-the core antigen (HCVcoreAg) and albumin). A selective experiment revealed a gradual increase in current responses in a HBsAg-spiked serum sample, demonstrating the interference-free detection of HBsAg for the real-life sample.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Aptamer; Sandwich Assay; Interdigitated Electrode Sensor; Biomarker; Gold Nanomaterial |
| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | Faculty of Dentistry > Department of Oral & Craniofacial Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 15 Jul 2024 08:21 |
| Last Modified: | 16 Jul 2024 07:27 |
| URI: | http://eprints.um.edu.my/id/eprint/40440 |
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