Hepatoprotective potential of clitoria ternatea Leaf extract against paracetamol induced damage in mice

Nithianantham, K. and Shyamala, M. and Chen, Y. and Latha, L.Y. and Jothy, S.L. and Sasidharan, S. (2011) Hepatoprotective potential of clitoria ternatea Leaf extract against paracetamol induced damage in mice. Molecules, 16 (12). pp. 10134-10145. ISSN 1420-3049

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Abstract

Background and Aim: Clitoria ternatea, a medicinal herb native to tropical equatorial Asia, is commonly used in folk medicine to treat various diseases. The aim of the present study is to evaluate the hepatoprotective and antioxidant activity of C. ternatea against experimentally induced liver injury. Methods: The antioxidant property of methanolic extract (ME) of C. ternatea leaf was investigated by employing an established in vitro antioxidant assay. The hepatoprotective effect against paracetamol-induced liver toxicity in mice of ME of C. ternatea leaf was also studied. Activity was measured by monitoring the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and billirubin along with histopathological analysis. Results: The amount of total phenolics and flavonoids were estimated to be 358.99 +/- 6.21 mg/g gallic acid equivalent and 123.75 +/- 2.84 mg/g catechin equivalent, respectively. The antioxidant activity of C. ternatea leaf extract was 67.85 at a concentration of 1 mg/mL and was also concentration dependant, with an IC(50) value of 420.00 mu g/mL. The results of the paracetamol-induced liver toxicity experiments showed that mice treated with the ME of C. ternatea leaf (200 mg/kg) showed a significant decrease in ALT, AST, and bilirubin levels, which were all elevated in the paracetamol group (p < 0.01). C. ternatea leaf extract therapy also protective effects against histopathological alterations. Histological studies supported the biochemical findings and a maximum improvement in the histoarchitecture was seen. Conclusions: The current study confirmed the hepatoprotective effect of C. ternatea leaf extract against the model hepatotoxicant paracetamol. The hepatoprotective action is likely related to its potent antioxidative activity.

Item Type: Article
Additional Information: ISI Document Delivery No.: 866UQ Times Cited: 0 Cited Reference Count: 28 Cited References: Adeneye AA, 2009, AFR J TRADIT COMPLEM, V6, P131 Aderogba M. A., 2004, Journal of Biological Sciences, V4, P501 Ajiboye TO, 2010, DRUG CHEM TOXICOL, V33, P77, DOI 10.3109/01480540903170746 Bhaskar VH, 2010, PHARM BIOL, V48, P1265, DOI 10.3109/13880201003730667 BHATHAL PS, 1983, BRIT J EXP PATHOL, V64, P524 Choi JH, 2009, J MED FOOD, V12, P320, DOI 10.1089/jmf.2007.0691 da Rocha RP, 2008, J BACTERIOL, V190, P1209, DOI 10.1128/JB.01419-07 Djeridane A, 2006, FOOD CHEM, V97, P654, DOI 10.1016/j.foodchem.2005.04.028 Fakurazi S, 2008, FOOD CHEM TOXICOL, V46, P2611, DOI 10.1016/j.fct.2008.04.018 Huang B, 2010, FOOD CHEM, V120, P873, DOI 10.1016/j.foodchem.2009.11.020 Iwalokun BA, 2006, J MED FOOD, V9, P524, DOI 10.1089/jmf.2006.9.524 KIRTIKAR KR, 1935, INDIAN MED PLANTS, P802 Malloy HT, 1937, J BIOL CHEM, V119, P481 Mukherjee PK, 2007, PHYTOTHER RES, V21, P1142, DOI 10.1002/ptr.2224 Mukherjeea PK, 2008, J ETHNOPHARMACOL, V120, P291, DOI 10.1016/j.jep.2008.09.009 Nayak SS, 2011, PHARM BIOL, V49, P111, DOI 10.3109/13880209.2010.501084 PATIL AP, 2011, INT J PHARM RES, V3, P20 Raghavendran HB, 2006, WORLD J GASTROENTERO, V12, P3829 RAO PG, 1993, INDIAN DRUGS, V30, P41 REITMAN S, 1957, AM J CLIN PATHOL, V28, P56 Sabir SM, 2008, J ETHNOPHARMACOL, V120, P226, DOI 10.1016/j.jep.2008.08.017 Sabir SM, 2008, FOOD CHEM, V111, P845, DOI 10.1016/j.foodchem.2008.04.060 Sasidharan S, 2010, MOLECULES, V15, P4478, DOI 10.3390/molecules15064478 SASIDHARAN S, 2007, INT J NAT ENG SCI, V1, P115 Sharma N, 2011, EXP TOXICOL PATHOL, V63, P671, DOI 10.1016/j.etp.2010.05.009 Yousef MI, 2010, FOOD CHEM TOXICOL, V48, P3246, DOI 10.1016/j.fct.2010.08.034 ZIMMERMANN M, 1983, PAIN, V16, P109, DOI 10.1016/0304-3959(83)90201-4 1995, INDIAN MED PLANTS, V2, P129 Nithianantham, Kuppan Shyamala, Murugesan Chen, Yeng Latha, Lachimanan Yoga Jothy, Subramanion L. Sasidharan, Sreenivasan Universiti Sains Malaysia from Institute for Postgraduate Studies, Universiti Sins Malaysia S. Jo Thy Lachumy was supported by Universiti Sains Malaysia fellowship from Institute for Postgraduate Studies, Universiti Sins Malaysia. Mdpi ag Basel
Uncontrolled Keywords: Clitoria ternatea paracetamol hepatoprotective antioxidant activity induced liver-damage in-vitro antioxidant induced hepatotoxicity aqueous extract medicinal-plants rats injury
Subjects: R Medicine > RK Dentistry
Divisions: Faculty of Dentistry
Depositing User: Ms Nursyafiqah Abd Malek
Date Deposited: 05 Dec 2012 03:41
Last Modified: 05 Dec 2012 03:41
URI: http://eprints.um.edu.my/id/eprint/4037

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