Bioassay-directed isolation of active compounds with antiyeast activity from a cassia fistula seed extract

Jothy, S.L.; Zakaria, Z.; Chen, Y.; Lau, Y.L.; Latha, L.Y.; Shin, L.N.; Sasidharan, S. (2011) Bioassay-directed isolation of active compounds with antiyeast activity from a cassia fistula seed extract. Molecules, 16 (9). pp. 7583-7592. ISSN 1420-3049

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      Abstract

      Background and objective: Cassia fistula L belongs to the family Leguminosae, and it is one of the most popular herbal products in tropical countries. C. fistula seeds have been used as a herbal medicine and have pharmacological activity which includes anti-bacterial, anti-fungal, and antioxidant properties. The goal of this study was to identify compounds from C. fistula seeds which are responsible for anti-Candida albicans activity using bioassay-directed isolation. Results: The preliminary phytochemical screening of the plant seed revealed the presence of anthraquinones, flavonoids, saponins, tannins and terpenoids. The isolation of active compounds was carried out in four steps: multiple extractions, fractionation using column chromatography and purification using preparative thin-layer chromatography (TLC) and liquid chromatography/mass spectrometry (LC/MS). The structure of separated compounds was determined on the basis of mass spectrometry data. One compound was identified is roseanone. Conclusions: The MS analysis on the active fraction from seed extract of C. fistula confirmed the presence of roseanone with antiyeast activity.

      Item Type: Article
      Creators:
      1. Jothy, S.L.
      2. Zakaria, Z.
      3. Chen, Y.
      4. Lau, Y.L.
      5. Latha, L.Y.
      6. Shin, L.N.
      7. Sasidharan, S.
      Journal or Publication Title: Molecules
      Additional Information: ISI Document Delivery No.: 824OI Times Cited: 0 Cited Reference Count: 16 Cited References: BORGERS M, 1989, J MED VET MYCOL, V27, P381 DINDA B, 1995, INDIAN J CHEM B, V34, P525 Duraipandiyan V, 2007, J ETHNOPHARMACOL, V112, P590, DOI 10.1016/j.jep.2007.04.008 Edeoga HO, 2005, AFR J BIOTECHNOL, V4, P685 Harborne J.B., 1998, PHYTOCHEMICAL METHOD, P40 HOMANS AL, 1970, J CHROMATOGR, V51, P327, DOI 10.1016/S0021-9673(01)96877-3 Kumar VP, 2006, J ETHNOPHARMACOL, V107, P182, DOI 10.1016/j.jep.2006.03.013 Miles RS, 1996, MACKIE MCCARTNEY PRA, P151 Newman DJ, 2007, J NAT PROD, V70, P461, DOI 10.1021/np068054v Newman DJ, 2003, J NAT PROD, V66, P1022, DOI 10.1021/np030096l POUCHUS YF, 1989, TETRAHEDRON COMPUTER, V2, P55, DOI 10.1016/0898-5529(89)90029-8 RAJAN S, 2001, ETHNOBOTANY, V6, P19 Sasidharan S, 2008, PHARM BIOL, V46, P261, DOI 10.1080/13880200701740858 Si WD, 2006, J CHROMATOGR A, V1125, P204, DOI 10.1016/j.chroma.2006.05.061 Tanaka N., 2001, ANAL CHEM, V73, P420 TOLSTIKOV VV, 2002, METABOLOMICS LC MS A Jothy, Subramanion L. Zakaria, Zuraini Chen, Yeng Lau, Yee Ling Latha, Lachimanan Yoga Shin, Lai Ngit Sasidharan, Sreenivasan Institute for Postgraduate Studies, Universiti Sins Malaysia S. Jo Thy Lachumy was supported by Universiti Sains Malaysia fellowship from Institute for Postgraduate Studies, Universiti Sins Malaysia. Mdpi ag Basel
      Uncontrolled Keywords: Bioactive compounds bioassay-directed isolation natural products extraction fractionation medicinal-plants natural-products antibacterial drugs
      Subjects: R Medicine > RK Dentistry
      Divisions: Faculty of Dentistry
      Depositing User: Ms Nursyafiqah Abd Malek
      Date Deposited: 05 Dec 2012 11:45
      Last Modified: 05 Dec 2012 11:45
      URI: http://eprints.um.edu.my/id/eprint/4036

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