Acute oral toxicity of methanolic seed extract of cassia fistula in mice

Jothy, S.L.; Zakaria, Z.; Chen, Y.; Lau, Y.L.; Latha, L.Y.; Sasidharan, S. (2011) Acute oral toxicity of methanolic seed extract of cassia fistula in mice. Molecules, 16 (6). pp. 5268-5282. ISSN 1420-3049

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      Abstract

      Background and objective: Cassia fistula is widely used in traditional medicine to treat various types of ailments. The evaluation of toxic properties of C. fistula is crucial when considering public health protection because exposure to plant extracts can result in undesirable effects on consumers. Hence, in this study the acute oral toxicity of C. fistula seeds extract was investigated in mice. Results: Oral administration of crude extract at the highest dose of 5000 mg/kg resulted in no mortalities or evidence of adverse effects, implying that C. fistula in nontoxic. Throughout 14 days of the treatment no changes in behavioural pattern, clinical sign and body weight of mice in both control and treatment groups. Also there were no any significant elevations observed in the biochemical analysis of the blood serum. Further, histopathological examination revealed normal architecture and no significant adverse effects observed on the kidney, heart, liver, lung and spleen. Conclusions: Overall, the results suggest that, the oral administration of C. fistula methanolic seeds extract did not produce any significant toxic effect in mice. Hence, the extract can be utilized for pharmaceutical formulations.

      Item Type: Article
      Creators:
      1. Jothy, S.L.
      2. Zakaria, Z.
      3. Chen, Y.
      4. Lau, Y.L.
      5. Latha, L.Y.
      6. Sasidharan, S.
      Journal or Publication Title: Molecules
      Additional Information: ISI Document Delivery No.: 782SW Times Cited: 0 Cited Reference Count: 43 Cited References: Adeneye AA, 2006, J ETHNOPHARMACOL, V105, P374, DOI 10.1016/j.jep.2005.11.027 AJAGBONNA OP, 1999, SOKOTO J VET SCI, V1, P3 Akanmu M. A., 2004, African Journal of Biomedical Research, V7, P23 Alade GO, 2009, AFR J PHARM PHARMACO, V3, P354 ALAM M M, 1990, Fitoterapia, V61, P240 ASANTEDUAH K, 2002, PUBLIC HLTH RISK ASS, V6 ASOLKAR LV, 1992, GLOSSARY INDIAN MED, V1, P177 Bellini MF, 2008, GENET MOL BIOL, V31, P122 BISWAS K, 1973, BHARATIA BANAWASADHI, V2, P336 Carol S.A., 1995, CRC HDB TOXICOLOGY, P51 Dybing E, 2002, FOOD CHEM TOXICOL, V40, P237, DOI 10.1016/S0278-6915(01)00115-6 Eaton DL, 1996, CASARETT DOULLS TOXI, P13 Harizal SN, 2010, J ETHNOPHARMACOL, V131, P404, DOI 10.1016/j.jep.2010.07.013 HUMBER JM, 2002, JAMA-J AM MED ASSOC, V288, P1655, DOI 10.1001/jama.288.13.1655 Raju Ilavarasan, 2005, African Journal of Traditional, Complementary and Alternative Medicines, V2, P70 IVERSEN PO, 2003, J NORW MED ASS, V123, P3402 Joshi CS, 2007, J HEALTH SCI, V53, P245, DOI 10.1248/jhs.53.245 KENNEDY GL, 1986, J APPL TOXICOL, V6, P145, DOI 10.1002/jat.2550060302 KIRTIKAR KR, 1975, INDIAN MED PLANTS, V2, P858 KLAASSEN CD, 2001, CASARETT DOULLS TOXI, P13 Kumar VP, 2006, J ETHNOPHARMACOL, V107, P182, DOI 10.1016/j.jep.2006.03.013 Lachumy S.J.T., 2010, RES J PHARM BIOL CHE, V1, P391 Markouk M, 2000, J ETHNOPHARMACOL, V73, P293, DOI 10.1016/S0378-8741(00)00257-9 Mcmanus J.G.A., 1984, STAINING METHODS HIS Moshi MJ, 2007, AFR J TRADIT COMPLEM, V4, P219 Newman DJ, 2003, J NAT PROD, V66, P1022, DOI 10.1021/np030096l OECD, 1995, OECD GUID TEST CHEM PATEL DG, 1965, ARCH INT PHARMACOD T, V157, P22 Perry LM, 1980, MED PLANTS E S E ASI RANG HP, 2001, PHARMACOLOGY, V13 Raza M., 2002, SCI PHARM, V70, P135 Rhiouani H, 2008, J ETHNOPHARMACOL, V118, P378, DOI 10.1016/j.jep.2008.05.009 Roopashree T. S., 2009, The Thai Journal of Pharmaceutical Sciences, V33, P74 Salawu OA, 2009, AFR J PHARM PHARMACO, V3, P621 SANGETHA, 2008, JAP J MED MYCOL, V49, P299 Sasidharan S, 2008, PHARM BIOL, V46, P413, DOI 10.1080/13880200802055867 SATYAVATI GV, 1989, MED PLANT INDIA Stevens Kent R., 1994, P673 Syahmi ARM, 2010, MOLECULES, V15, P8111, DOI 10.3390/molecules15118111 Teo S, 2002, TOXICOLOGY, V179, P183, DOI 10.1016/S0300-483X(02)00338-4 Vaghasiya Y. K., 2011, Journal of Pharmacology and Toxicology, V6, P113, DOI 10.3923/jpt.2011.113.123 WALUM E, 1995, ALTERNATIVE METHODS, V11, P275 *OECD, 2001, 420 OECD Jothy, Subramanion L. Zakaria, Zuraini Chen, Yeng Lau, Yee Ling Latha, Lachimanan Yoga Sasidharan, Sreenivasan Institute for Postgraduate Studies, Universiti Sins Malaysia S. Jo Thy Lachumy was supported by Universiti Sains Malaysia fellowship from Institute for Postgraduate Studies, Universiti Sins Malaysia. Mdpi ag Basel
      Uncontrolled Keywords: Cassia fistula methanol extract acute oral toxicity histology hematology subacute toxicity aqueous extract rats plants leaf
      Subjects: R Medicine > RK Dentistry
      Divisions: Faculty of Dentistry
      Depositing User: Ms Nursyafiqah Abd Malek
      Date Deposited: 05 Dec 2012 11:49
      Last Modified: 05 Dec 2012 11:49
      URI: http://eprints.um.edu.my/id/eprint/4035

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