Prevalence of microsporidia in an indigenous Orang Asli community in Pahang, Malaysia

Lono, A.; Kumar, G.S.; Chye, T.T. (2010) Prevalence of microsporidia in an indigenous Orang Asli community in Pahang, Malaysia. Transactions of the Royal Society of Tropical Medicine and Hygiene, 104 (3). pp. 214-218. ISSN 0035-9203

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    Abstract

    Microsporidia are ubiquitous parasites thought to be closely related to fungi. Their presence in the environment means that humans are frequently exposed to infection. Stool samples were collected from 151 indigenous villagers from the eastern state of Pahang in 2005. The samples were concentrated with water-ether sedimentation, stained with modified trichrome stain and examined under oil-immersion microscopy. Thirty-two specimens (21.2) were positive for microsporidia. Microsporidia were observed as ovoid or rounded ovoid shapes measuring similar to 1 mu m, with a bright pink outline containing a central or posterior vacuole. PCR amplification with specific primers on microscopy-positive specimens amplified Encephalitozoon intestinalis DNA from five of the ten specimens used. (C) 2009 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.

    Item Type: Article
    Creators:
    1. Lono, A.
    2. Kumar, G.S.
    3. Chye, T.T.
    Journal or Publication Title: Transactions of the Royal Society of Tropical Medicine and Hygiene
    Additional Information: Lono, Angela Kumar, Govind Suresh Chye, Tan Tian
    Uncontrolled Keywords: Microsporidia;Parasites;Encephalitozoon intestinalis;Microscopy;PCR;Malaysia
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Ms Maisarah Mohd Muksin
    Date Deposited: 03 Dec 2012 09:11
    Last Modified: 03 Dec 2012 09:11
    URI: http://eprints.um.edu.my/id/eprint/4028

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