Azlan, Ummi Wahidah and Lau, Yee Ling and Fong, Mun Yik (2022) Genetic diversity and clustering of the rhoptry associated Protein-1 of Plasmodium knowlesi from Peninsular Malaysia and Malaysian Borneo. Korean Journal of Parasitology, 60 (6). pp. 393-400. ISSN 0023-4001, DOI https://doi.org/10.3347/kjp.2022.60.6.393.
Full text not available from this repository.Abstract
Human infection with simian malaria Plasmodium knowlesi is a cause for concern in Southeast Asian countries, especially in Malaysia. A previous study on Peninsular Malaysia P. knowlesi rhoptry associated protein-1 (PkRAP1) gene has discovered the existence of dimorphism. In this study, genetic analysis of PkRAP1 in a larger number of P. knowlesi samples from Malaysian Borneo was conducted. The PkRAP1 of these P. knowlesi isolates was PCR-amplified and se-quenced. The newly obtained PkRAP1 gene sequences (n= 34) were combined with those from the previous study (n= 26) and analysed for polymorphism and natural selection. Sequence analysis revealed a higher genetic diversity of PkRAP1 compared to the previous study. Exon II of the gene had higher diversity (n= 0.0172) than exon I (n= 0.0128). The diversity of the total coding region (n= 0.0167) was much higher than those of RAP1 orthologues such as PfRAP-1 (n= 0.0041) and PvRAP1 (n= 0.00088). Z-test results indicated that the gene was under purifying selection. Phylogenetic tree and haplotype network showed distinct clustering of Peninsular Malaysia and Malaysian Borneo PkRAP1 haplotypes. This geographical-based clustering of PkRAP1 haplotypes provides further evidence of the dimorphism of the gene and possible existence of 2 distinct P. knowlesi lineages in Malaysia.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Education, Malaysia (Grant No: LRGS/1/2018/UM/01/1/1) |
| Uncontrolled Keywords: | Plasmodium knowlesi; Rhoptry associated protein-1; Genetic diversity |
| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | Faculty of Medicine > Parasitology Deparment |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 20 Nov 2023 09:20 |
| Last Modified: | 20 Nov 2023 09:20 |
| URI: | http://eprints.um.edu.my/id/eprint/40271 |
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