Al-Trad, Esra'a and Hamzah, Ainal Mardziah Che and Puah, Suat Moi and Chua, Kek Heng and Kwong, Stephen M. and Yeo, Chew Chieng and Chew, Ching Hoong (2022) Comparative genomic analysis of a multidrug-resistant Staphylococcus hominis ShoR14 clinical isolate from Terengganu, Malaysia, led to the discovery of novel mobile genetic elements. Pathogens, 11 (12). ISSN 2076-0817, DOI https://doi.org/10.3390/pathogens11121406.
Full text not available from this repository.Abstract
Staphylococcus hominis is a coagulase-negative Staphylococcus (CoNS) commensal capable of causing serious systemic infections in humans. The emergence of multidrug-resistant S. hominis strains is of concern but little is known about the characteristics of this organism, particularly from Malaysia. Here, we present the comparative genome analysis of S. hominis ShoR14, a multidrug-resistant, methicillin-resistant blood isolate from Terengganu, Malaysia. Genomic DNA of S. hominis ShoR14 was sequenced on the Illumina platform and assembled using Unicycler v0.4.8. ShoR14 belonged to sequence type (ST) 1 which is the most prevalent ST of the S. hominis subsp. hominis. Comparative genomic analysis with closely related strains in the database with complete genome sequences, led to the discovery of a novel variant of the staphylococcal chromosome cassette mec (SCCmec) type VIII element harboring the mecA methicillin-resistance gene in ShoR14 and its possible carriage of a SCCfus element that encodes the fusidic acid resistance gene (fusC). Up to seven possible ShoR14 plasmid contigs were identified, three of which harbored resistance genes for tetracycline (tetK), chloramphenicol (catA7), macrolides, lincosamides, and streptogramin B (ermC). Additionally, we report the discovery of a novel mercury-resistant transposon, Tn7456, other genomic islands, and prophages which make up the S. hominis mobilome.
| Item Type: | Article |
|---|---|
| Funders: | None |
| Uncontrolled Keywords: | Staphylococcus hominis; Multidrug resistance; Whole genome sequencing; Genomic islands; Plasmids; Prophages |
| Subjects: | Q Science > QH Natural history > QH426 Genetics Q Science > QR Microbiology |
| Divisions: | Faculty of Medicine > Biomedical Science Department |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 24 Nov 2023 07:54 |
| Last Modified: | 24 Nov 2023 07:54 |
| URI: | http://eprints.um.edu.my/id/eprint/40268 |
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