Enterovirus 71 Infection Induces Apoptosis in Vero Cells

Chan, Y.F.; Abubakar, S. (2003) Enterovirus 71 Infection Induces Apoptosis in Vero Cells. Malaysian Journal of Pathology, 25 (1). pp. 29-35. ISSN 0126-8635 (Print) 0126-8635 (Linking)

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    Abstract

    The effects of Enterovirus 71 (HEV71) infection on African green monkey kidney cells (Vero) were investigated. It was found that the infected cells showed progressive cellular morphological changes characteristic in apoptotic cells within 10 hours post-infection. The number of apoptotic cells correlated significantly with the number of HEV71 antigen positive cells when cells were labeled using terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) and stained for HEV71 antigen. Approximately 11, 26, 45 and 50 of the infected cells were apoptotic at 12, 24, 48 and 72 hours post-infection, respectively. Internucleosomal DNA fragmentation, characteristic in the late stage of apoptosis was noted beginning on day 2 post-infection. The DNA fragmentation, however, was absent in cells treated with the heat- and ultraviolet light-inactivated virus inocula. These results demonstrate the capacity of HEV71 to induce apoptosis in the infected cells. The induction, however, requires high level of HEV71 infectivity and the presence of live virus particles, suggesting the need for the presence of specific viral proteins for apoptosis to occur.

    Item Type: Article
    Creators:
    1. Chan, Y.F.
    2. Abubakar, S.(Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.)
    Journal or Publication Title: Malaysian Journal of Pathology
    Additional Information: Chan, Yoke-Fun Abubakar, Sazaly eng Research Support, Non-U.S. Gov't Malaysia 2005/10/04 09:00 Malays J Pathol. 2003 Jun;25(1):29-35.
    Uncontrolled Keywords: Animals Apoptosis/*physiology Cercopithecus aethiops Cytopathogenic Effect, Viral/physiology Enterovirus D, Human/*physiology Enterovirus Infections/*pathology Humans In Situ Nick-End Labeling Vero Cells
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: miss munirah saadom
    Date Deposited: 27 Nov 2012 10:15
    Last Modified: 27 Nov 2012 10:15
    URI: http://eprints.um.edu.my/id/eprint/3999

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