Effects of Dengue 2 Virus inoculation on Toxorhynchites splendens larvae

Shafee, N.; AbuBakar, S. (2006) Effects of Dengue 2 Virus inoculation on Toxorhynchites splendens larvae. Journal of Entomology, 3. pp. 89-94.

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    Abstract

    Potential occurrence of dengue 2 virus (DENV-2)-induced apoptosis in mosquito larvae was investigated. Heads of Toxohrynchites splendens mosquito larvae were inoculated with DENV-2 inoculum at multiplicity of infection of 0.0005-0.001 plaque forming unit per cell. After 7 days of incubation, the larvae heads were severed and squashed onto slides. Following immunofluorescence staining with mouse polyclonal antibody against DENV-2, antigens were detected in 17.5 of the total cells counted. Counterstaining of the cells using colorimetric apoptotic staining method revealed that 21.7 of the antigen-positive cells were in apoptotic state. Statistical analyses of the data showed that the induction of apoptosis was directly correlated to the DENV-2 infection and not merely a metamorphic programmed cell death response.

    Item Type: Article
    Creators:
    1. Shafee, N.
    2. AbuBakar, S.(University of Malaya)
    Journal or Publication Title: Journal of Entomology
    Uncontrolled Keywords: Dengue fever; Toxohrynchites; Mosquito larvae; immunofluorescence staining
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Miss Nur Jannatul Adnin Ahmad Shafawi
    Date Deposited: 28 Nov 2012 11:46
    Last Modified: 28 Nov 2012 11:46
    URI: http://eprints.um.edu.my/id/eprint/3994

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