Dengue Virus Type 2 NS3 Protease and NS2B-NS3 Protease Precursor induce Apoptosis

Shafee, N.; AbuBakar, S. (2003) Dengue Virus Type 2 NS3 Protease and NS2B-NS3 Protease Precursor induce Apoptosis. Journal of General Virology, 84. pp. 2191-2195. ISSN 0022-1317

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    Abstract

    Apoptosis was detected in Vero cell cultures expressing transfected dengue virus type 2 (DENV-2) genes. Approximately 17(.)5 and 51(.)5 of cells expressing NS3 serine protease and NS2B-NS3(185) serine protease precursor protein NS2B-NS3(185)(pro) genes, respectively, were apoptotic. The percentage of apoptotic cells was significantly higher in cell cultures expressing NS2B-NS3(185)(pro). NS2B-NS3(185)(pro) was detected as NS2B-NS3(185)(pro)-EGFP fusion protein in cytoplasmic vesicular structures in the apoptotic cells. Site-directed mutagenesis which replaced His 51 with Ala within the protease catalytic triad significantly reduced the ability of the expressed NS3 and NS2B-NS3(185)(pro) to induce apoptosis. Results from the present study showed that DENV-2-encoded NS3 serine protease induces apoptosis, which is enhanced in cells expressing its precursor, NS2B-NS3(185)(pro). These findings suggest the importance of NS2B as a cofactor to NS3 protease-induced apoptosis.

    Item Type: Article
    Creators:
    1. Shafee, N.
    2. AbuBakar, S.(Department of Medical Microbiology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    Journal or Publication Title: Journal of General Virology
    Additional Information: Shafee, N AbuBakar, S Part 8
    Uncontrolled Keywords: Amino Acid Sequence Animals *Apoptosis Cercopithecus aethiops Dengue Virus/*pathogenicity Green Fluorescent Proteins Humans Luminescent Proteins/genetics/metabolism Mice Molecular Sequence Data Protein Precursors/*metabolism RNA Helicases Recombinant Fusion Proteins/genetics/metabolism Serine Endopeptidases Vero Cells Viral Nonstructural Proteins/chemistry/*metabolism
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: miss munirah saadom
    Date Deposited: 29 Nov 2012 09:53
    Last Modified: 29 Nov 2012 09:53
    URI: http://eprints.um.edu.my/id/eprint/3990

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