Wong, P.F. and AbuBakar, Sazaly (2008) High intracellular Zn(2+) ions modulate the VHR, ZAP-70 and ERK activities of LNCaP prostate cancer cells. Cellular & Molecular Biology Letters, 13 (3). pp. 375-390. ISSN 1689-1392, DOI 18311544.
Full text not available from this repository.Abstract
Malignant prostate tissues have markedly reduced zinc (Zn(2+)) contents in comparison to non-malignant tissues. In this study, we restored a high intracellular Zn(2+) level to LNCaP prostate cancer cells by culturing the cells in a growth medium supplemented with a supraphysiological concentration of Zn(2+) (10 mu g/ml) over 5 weeks. The intracellular Zn(2+) level increased in the Zn(2+)-treated cells, and there was a marked increase in the presence of zincosomes, a Zn(2+)-specific intracellular organelle. The proliferation rate of the Zn(2+)-treated cells was markedly reduced. There was also a significant increase (36.6 +/- 6.4) in the total tyrosine phosphorylated proteins. Vaccinia H1-related (VHR) phosphatase, zeta chain-associated protein-70 (ZAP-70) kinase and phosphorylated extracellular signal-regulated protein kinase 1 and 2 (p-ERK 1 and 2) were also present in higher abundance. Treatment with TPEN, which chelates Zn(2+), reduced the abundance of VHR phosphatase and ZAP-70 kinase, but increased the abundance of p-ERK 1. However, the TPEN treatment restored the Zn(2+)-treated LNCaP cell proliferation to a rate comparable to that of the non Zn(2+)-treated cells. These results highlight the importance of a high intracellular Zn(2+) content and the VHR/ZAP-70-associated pathways in the modulation of LNCaP prostate cancer cell growth.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Additional Information: | Wong, Pooi-Fong Abubakar, Sazaly eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Poland 2008/03/04 09:00 Cell Mol Biol Lett. 2008;13(3):375-90. Epub 2008 Feb 29. |
| Uncontrolled Keywords: | Animals Cell Line, Tumor Cell Proliferation Chelating Agents/metabolism Culture Media/chemistry Dual Specificity Phosphatase 3/*metabolism Ethylenediamines/metabolism Extracellular Signal-Regulated MAP Kinases/*metabolism Humans Male Prostatic Neoplasms/*metabolism Protein Tyrosine Phosphatases/metabolism Protein-Tyrosine Kinases/metabolism ZAP-70 Protein-Tyrosine Kinase/*metabolism Zinc/*metabolism |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Mr Jenal S |
| Date Deposited: | 29 Nov 2012 01:57 |
| Last Modified: | 13 Feb 2019 08:10 |
| URI: | http://eprints.um.edu.my/id/eprint/3969 |
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