In vitro antiviral activity of Fisetin, Rutin and Naringenin against Dengue virus type-2

Zandi, K.; Teoh, B.T.; Sam, S.S.; Wong, P.F.; Mustafa, M.R.; AbuBakar, S. (2011) In vitro antiviral activity of Fisetin, Rutin and Naringenin against Dengue virus type-2. Journal of Medicinal Plants Research, 5 (23). pp. 5534-5539. ISSN 1996-0875

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In vitro antiviral activities of three flavonoids; fisetin, naringenin and rutin against DENV-2 (NGC strain) were evaluated. Inhibitory effects of each compound at the different stages of DENV-2 infection were examined using foci forming unit reduction assay (FFURA) and quantitative real-time polymerase chain amplification (qRT-PCR). Fisetin, rutin and naringenin showed cytotoxic effects against Vero cells with 50 cytotoxicity (CC(50)) values of 247, > 1000, and 87 mu g/mL, respectively. Fisetin when added to Vero cells after virus adsorption inhibited DENV replication with a half maximal inhibition concentration (IC(50)) value of 55 mu g/mL and selectivity index (SI) of 4.49. The IC(50) value of fisetin was 43.12 mu g/mL with SI=5.72 when Vero cells were treated for 5 h before virus infection and continuously up to 4 days post-infection. There was no direct virucidal activity or prophylactic activity of fisetin against DENV-2. Rutin and naringenin did not inhibit DENV-2 replication in Vero cells. Naringenin however, exhibited direct virucidal activity against DENV-2 with IC(50) = 52.64 mu g/mL but the SI was <1. The present study suggests that among the flavonoids examined, only fisetin showed significant in vitro anti dengue virus replication activity.

Item Type: Article
  1. Zandi, K.
  2. Teoh, B.T.
  3. Sam, S.S.
  4. Wong, P.F.
  5. Mustafa, M.R.
  6. AbuBakar, S.(Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
Journal or Publication Title: Journal of Medicinal Plants Research
Additional Information: Zandi, Keivan Teoh, Boon-Teong Sam, Sing-Sin Wong, Pooi-Fong Mustafa, Mohd Rais AbuBakar, Sazaly
Uncontrolled Keywords: Infectious disease, virology, dengue, antivirals, flavonoid
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms Maisarah Mohd Muksin
Date Deposited: 12 Nov 2012 09:27
Last Modified: 07 Nov 2017 16:36

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