Ng, Yee Ling and Lau, Yee Ling and Hamid, Mohd Hafizi Abdul and Jelip, Jenarun and Ooi, Choo Huck and Mudin, Rose Nani and Jaimin, Joel Judson and Fong, Mun Yik (2023) Genetic polymorphism of the thrombospondin-related apical merozoite protein (TRAMP) of Plasmodium knowlesi in Malaysia. Parasitology Research, 122 (1). pp. 195-200. ISSN 0932-0113, DOI https://doi.org/10.1007/s00436-022-07716-z.
Full text not available from this repository.Abstract
Plasmodium knowlesi is a simian malaria parasite that causes significant zoonotic infections in Southeast Asia, particularly in Malaysia. The Plasmodium thrombospondin-related apical merozoite protein (TRAMP) plays an essential role in the invasion of the parasite into its host erythrocyte. The present study investigated the genetic polymorphism and natural selection of the full length PkTRAMP from P. knowlesi clinical isolates from Malaysia. Blood samples (n=40) were collected from P. knowlesi malaria patients from Peninsular Malaysia and Malaysian Borneo. The PkTRAMP gene was amplified using PCR, followed by cloning into a plasmid vector and sequenced. Results showed that the nucleotide diversity of PkTRAMP was low (pi: 0.009). Z-test results indicated negative (purifying) selection of PkTRAMP. The alignment of the deduced amino acid sequences of PkTRAMP of Peninsular Malaysia and Malaysian Borneo revealed 38 dimorphic sites. A total of 27 haplotypes were identified from the amino acid sequence alignment. Haplotype analysis revealed that there was no clustering of PkTRAMP from Peninsular Malaysia and Malaysian Borneo.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Higher Education Malaysia Long Term Research Grant Scheme (Grant No: LRGS/1/2018/UM/01/1/1) |
| Uncontrolled Keywords: | Plasmodium knowlesi; Thrombospondin-related apical merozoite protein; Genetic polymorphism; Haplotypes |
| Subjects: | Q Science > QH Natural history > QH426 Genetics |
| Divisions: | Faculty of Medicine > Parasitology Deparment |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 29 Nov 2023 01:16 |
| Last Modified: | 29 Nov 2023 01:16 |
| URI: | http://eprints.um.edu.my/id/eprint/39328 |
Actions (login required)
 |
View Item |
