Identification of a 48 kDa tubulin or tubulin-like C6/36 mosquito cells protein that binds dengue virus 2 using mass spectrometry

Chee, H.Y.; AbuBakar, S. (2004) Identification of a 48 kDa tubulin or tubulin-like C6/36 mosquito cells protein that binds dengue virus 2 using mass spectrometry. Biochemical and Biophysical Research Communications, 320 (1). pp. 11-17. ISSN 0006-291X

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    Abstract

    Binding of dengue virus 2 (DENV-2) to C6/36 mosquito cells protein was investigated. A 48 kDa DENV-2-binding C6/36 cells protein (D2BP) was detected in a virus overlay protein-binding assay. The binding occurred only to the C6/36 cells cytosolic protein fraction and it was inhibited by free D2BP. D2BP was shown to bind to DENV-2 E in the far-Western-binding studies and using mass spectrometry (MS) and MS/MS, peptide masses of the D2BP that matched to beta-tubulin and alpha-tubulin chains were identified. These findings suggest that DENV-2 through DENV-2 E binds directly to a 48 kDa tubulin or tubulin-like protein of C6/36 mosquito cells. (C) 2004 Elsevier Inc. All rights reserved.

    Item Type: Article
    Journal or Publication Title: Biochemical and Biophysical Research Communications
    Additional Information: Chee, HY AbuBakar, S
    Uncontrolled Keywords: Amino Acid Sequence Animals Binding Sites Cell Line Culicidae/*metabolism/*virology Dengue Virus/*metabolism Insect Proteins/*chemistry/metabolism Molecular Sequence Data Molecular Weight Protein Binding Protein Interaction Mapping/*methods Sequence Alignment Sequence Homology, Amino Acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods Tubulin/*chemistry/metabolism Viral Envelope Proteins/*chemistry/metabolism
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Ms Johana Johari
    Date Deposited: 08 Nov 2012 12:05
    Last Modified: 08 Nov 2012 12:05
    URI: http://eprints.um.edu.my/id/eprint/3923

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