Ng, Yee Ling and Lee, Wenn Chyau and Lau, Yee Ling and Fong, Mun Yik (2023) The impact of geographical variation in Plasmodium knowlesi Apical Membrane Protein 1 (PkAMA-1) on invasion dynamics of P. knowlesi. Tropical Medicine and Infectious Disease, 8 (1). ISSN 2414-6366, DOI https://doi.org/10.3390/tropicalmed8010056.
Full text not available from this repository.Abstract
Plasmodium knowlesi has emerged as an important zoonotic parasite that causes persistent symptomatic malaria in humans. The signs and symptoms of malaria are attributed to the blood stages of the parasites, which start from the invasion of erythrocytes by the blood stage merozoites. The apical membrane protein 1 (AMA-1) plays an important role in the invasion. In this study, we constructed and expressed recombinant PkAMA-1 domain II (PkAMA-1-DII) representing the predominant haplotypes from Peninsular Malaysia and Malaysian Borneo and raised specific antibodies against the recombinant proteins in rabbits. Despite the minor amino acid sequence variation, antibodies raised against haplotypes from Peninsular Malaysia and Malaysian Borneo demonstrated different invasion inhibition (46.81% and 39.45%, respectively) to P. knowlesi A1-H.1, a reference strain derived from Peninsular Malaysia. Here, we demonstrated how a minor variation in a conserved parasite protein could cast a significant impact on parasite invasion biology, suggesting a complex host-switching of P. knowlesi from different locations. This may challenge the implementation of a standardized One Health approach against the transmission of knowlesi malaria.
| Item Type: | Article |
|---|---|
| Funders: | Long Term Research Grant Scheme (LRGS) of the Ministry of Higher Education, Malaysia (Grant No: LRGS/1/2018/UM/01/1/1), Ministry of Education, Malaysia (Grant No: LRGS/1/2018/UM/01/1/4), Fundamental Research Grant Scheme (FRGS) of the Ministry of Higher Education, Malaysia (Grant No: FRGS/1/2022/SKK12/UM/02/9) |
| Uncontrolled Keywords: | Plasmodium knowlesi; PkAMA-1; Merozoite invasion; Peninsular Malaysia; Malaysian Borneo |
| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | Faculty of Medicine > Parasitology Deparment |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 30 Nov 2023 02:48 |
| Last Modified: | 30 Nov 2023 02:48 |
| URI: | http://eprints.um.edu.my/id/eprint/39066 |
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