Alshehade, Salah Abdalrazak and Al Zarzour, Raghdaa Hamdan and Mathai, Michael and Giribabu, Nelli and Seyedan, Atefehalsadat and Kaur, Gurjeet and Al-Suede, Fouad Saleih Resq and Majid, Amin Malik Shah Abdul and Murugaiyah, Vikneswaran and Almoustafa, Hassan and Alshawsh, Mohammed Abdullah (2023) Orthosiphon aristatus (Blume) Miq Alleviates Non-Alcoholic Fatty Liver Disease via Antioxidant Activities in C57BL/6 Obese Mice and Palmitic-Oleic Acid-Induced Steatosis in HepG2 Cells. PHARMACEUTICALS, 16 (1).
Full text not available from this repository.Abstract
Non-alcoholic fatty liver disease (NAFLD) is the most prevalent form of liver disease. Orthosiphon aristatus (Blume) Miq, a traditional plant in South Asia, has previously been shown to attenuate obesity and hyperglycaemic conditions. Eight weeks of feeding C57BL/6 mice with the standardized O. aristatus extract (400 mg/kg) inhibited the progression of NAFLD. Liver enzymes including alanine aminotransferase and aspartate transaminase were significantly reduced in treated mice by 74.2% +/- 7.69 and 52.8% +/- 7.83, respectively. Furthermore, the treated mice showed a reduction in serum levels of glucose (50% +/- 5.71), insulin (70.2% +/- 12.09), total cholesterol (27.5% +/- 15.93), triglycerides (63.2% +/- 16.5), low-density lipoprotein (62.5% +/- 4.93) and atherogenic risk index relative to the negative control. Histologically, O. aristatus reversed hepatic fat accumulation and reduced NAFLD severity. Notably, our results showed the antioxidant activity of O. aristatus via increased superoxide dismutase activity and a reduction of hepatic malondialdehyde levels. In addition, the levels of serum pro-inflammatory mediators (IL-6 and TNF alpha) decreased, indicating anti-inflammatory activity. The aqueous, hydroethanolic and ethanolic fractions of O. aristatus extract significantly reduced intracellular fat accumulation in HepG2 cells that were treated with palmitic-oleic acid. Together, these findings suggest that antioxidant activities are the primary mechanism of action of O. aristatus underlying the anti-NAFLD effects.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Orthosiphon aristatus; non-alcoholic fatty liver disease; atherosclerosis; medicinal plant; high-fat diet; rosmarinic acid |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 27 Nov 2024 04:09 |
| Last Modified: | 27 Nov 2024 04:09 |
| URI: | http://eprints.um.edu.my/id/eprint/38948 |
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