Nam, Hui Yin and Yusof, Mohd Rusdi and Kamarul, Tunku (2023) Adipose and bone marrow derived-mesenchymal stromal cells express similar tenogenic expression levels when subjected to mechanical uniaxial stretching in vitro. Stem Cells International, 2023. ISSN 1687-966X, DOI https://doi.org/10.1155/2023/4907230.
Full text not available from this repository.Abstract
The present study was conducted to determine whether adipose derived mesenchymal stromal cells (AD-MSCs) or bone marrow derived-MSCs (BM-MSCs) would provide superior tenogenic expressions when subjected to cyclical tensile loading. The results for this would indicate the best choice of MSCs source to be used for cell-based tendon repair strategies. Both AD-MSCs and BM-MSCs were obtained from ten adult donors (N=10) and cultured in vitro. At passaged-2, cells from both groups were subjected to cyclical stretching at 1 Hz and 8% of strain. Cellular morphology, orientation, proliferation rate, protein, and gene expression levels were compared at 0, 24, and 48 hours of stretching. In both groups, mechanical stretching results in similar morphological changes, and the redirection of cell alignment is perpendicular to the direction of stretching. Loading at 8% strain did not significantly increase proliferation rates but caused an increase in total collagen expression and tenogenic gene expression levels. In both groups, these levels demonstrated no significant differences suggesting that in a similar loading environment, both cell types possess similar tenogenic potential. In conclusion, AD-MSCs and BM-MSCs both demonstrate similar tenogenic phenotypic and gene expression levels when subjected to cyclic tensile loading at 1 Hz and 8% strain, thus, suggesting that the use of either cell source may be suitable for tendon repair.
| Item Type: | Article |
|---|---|
| Funders: | Fundamental Research Grant Scheme (FRGS) from the Ministry of Higher Education, Malaysia (FRGS/1/2018/STG04/UM/02/12 (FP033-2018A)), Universiti Malaya Faculty Research Grant (GPF003C-2018) |
| Uncontrolled Keywords: | Adipose; Bone marrow; Derived-mesenchymal stromal cells; Tenogenic expression levels; Mechanical uniaxial stretching |
| Subjects: | Q Science > Q Science (General) R Medicine |
| Divisions: | Faculty of Medicine > Orthopaedic Surgery Department Deputy Vice Chancellor (Research & Innovation) Office > Nanotechnology & Catalysis Research Centre |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 06 Jul 2023 04:47 |
| Last Modified: | 06 Jul 2023 04:47 |
| URI: | http://eprints.um.edu.my/id/eprint/38765 |
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