Hypotensive effect of Gentiana floribunda is mediated through Ca antagonism pathway

Khan, A.; Mustafa, M.R.; Khan, A.U.; Murugan, D.D. (2012) Hypotensive effect of Gentiana floribunda is mediated through Ca antagonism pathway. BMC Complementary and Alternative Medicine, 12. p. 121. ISSN 1472-6882

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    Abstract

    Background: Gentiana floribunda was investigated for the possible hypotensive and vasodilator activities in an attempt to rationalize its traditional use in hypertension. Methods: The crude extract of Gentiana floribunda (Gf.Cr) was studied in anaesthetized rats and isolated thoracic aorta tissues. Results: Gf.Cr which tested positive for presence of flavonoids, saponins, sterols, tannins and terpenes caused dose-dependent (3.0-100 mg/kg) fall in arterial blood pressure (BP) of rats under anaesthesia. In rat aortic ring preparations denuded of endothelium, Gf.Cr at concentration range of 1.0-10 mg/mL relaxed high K+ (80 mM) and phenylephrine (PE, 1 muM)-induced contractions and shifted Ca++ dose--response curves to right, similar to that caused by verapamil. It also suppressed PE (1 muM) control peak responses at 0.3-1.0 mg/mL, obtained in Ca++-free medium, as exhibited by verapamil. Pre-treatment of tissues with Gf.Cr produced rightward non-parallel shift of PE-curves with decline of maximum contractile response. The vasodilator effect of Gf.Cr was endothelial-independent, as it was not blocked by Nomega-nitro-L-arginine methyl ester hydrochloride, atropine and indomethacin in endothelium-intact aortic tissues. Conclusions: These data indicate that BP-lowering action of Gentiana floribunda occurred via Ca++ antagonism (inhibition of Ca++ ingress and release from intracellular stores), which provides pharmacological basis to justify its effectiveness in hypertension.

    Item Type: Article
    Creators:
    1. Khan, A.
    2. Mustafa, M.R.(Department of Pharmacology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    3. Khan, A.U.
    4. Murugan, D.D.
    Journal or Publication Title: BMC Complementary and Alternative Medicine
    Additional Information: Department of Pharmacology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
    Uncontrolled Keywords: Pharmacology
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Ms Haslinda Lahuddin
    Date Deposited: 02 Nov 2012 09:28
    Last Modified: 02 Nov 2012 09:28
    URI: http://eprints.um.edu.my/id/eprint/3874

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