Supramani, Sugenendran and Rejab, Nur Ardiyana and Ilham, Zul and Ahmad, Rahayu and Show, Pau-Loke and Ibrahim, Mohamad Faizal and Wan-Mohtar, Wan Abd Al Qadr Imad (2023) Performance of biomass and exopolysaccharide production from the medicinal mushroom ganoderma lucidum in a new fabricated Air-L-Shaped Bioreactor (ALSB). Processes, 11 (3). ISSN 2227-9717, DOI https://doi.org/10.3390/pr11030670.
Full text not available from this repository.Abstract
Conventional stirred-tank bioreactor (STR) designs are optimised for cultures of bacteria but not fungal cultures; therefore, a new Air-L-Shaped Bioreactor (ALSB) was fabricated. The ALSB was designed to eliminate the wall growth and clumping of fungal mycelium in STRs. Ganoderma lucidum was used as a fungal model and its biomass and exopolysaccharide (EPS) production were maximised by optimising the agitation rate, glucose concentration, initial pH, and aeration via response surface methodology (RSM). The ALSB system generated 7.8 g/L of biomass (biomass optimised conditions: 110 rpm, 24 g/L glucose, pH 5.6, and 3 v/v of aeration) and 4.4 g/L of EPS (EPS optimised conditions: 90 rpm, 30 g/L glucose, pH 4, and 2.5 v/v of aeration). In combination, for both optimised conditions, biomass (7.9 g/L) and EPS (4.6 g/L) were produced at 110 rpm, 30 g/L glucose, pH 4, and 3 v/v of aeration with minimal wall growth. The data prove that the ALSB is a blueprint for efficient economical fungal cultivation.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Ganoderma lucidum; Smart bioreactor; Bioprocess optimization; Wall growth; Exopolysaccharide |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Science Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 01 Nov 2024 03:03 |
| Last Modified: | 01 Nov 2024 03:03 |
| URI: | http://eprints.um.edu.my/id/eprint/38558 |
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