Ranjutha, Valiappan and Chen, Yeng and Al-Keridis, Lamya Ahmed and Patel, Mitesh and Alshammari, Nawaf and Adnan, Mohd and Sahreen, Sumaira and Gopinath, Subash C. B. and Sasidharan, Sreenivasan (2023) Synergistic antimicrobial activity of Ceftriaxone and Polyalthia longifolia Methanol (MEPL) leaf extract against Methicillin-Resistant Staphylococcus aureus and modulation of mecA gene presence. Antibiotics-Basel, 12 (3). ISSN 2079-6382, DOI https://doi.org/10.3390/antibiotics12030477.
Full text not available from this repository.Abstract
Medicinal plants are an essential source of traditional curatives for numerous skin diseases. Polyalthia longifolia (Sonn.) Thwaites (Annonaceae family) is a medicinal plant used to cure skin illnesses. P. longifolia is usually applied in folkloric therapeutical systems to treat skin diseases. The methicillin-resistant Staphylococcus aureus (MRSA) bacteria is among the essential bacteria contributing to skin diseases. Hence, to verify the traditional medicinal claim of P. longifolia usage in skin disease treatment, the current research was performed to study the synergistic antibacterial activity of standardized Polyalthia longifolia methanol leaf extract (MEPL) against MRSA bacteria. The synergistic antimicrobial activity result of ceftriaxone, when mixed with MEPL, against MRSA was investigated by the disc diffusion method, broth microdilution method, checkerboard dilution test, and modulation of mecA gene expression by multiplex polymerase chain reaction (multiplex PCR). The MEPL extract exhibited good synergistic antimicrobial activity against MRSA. Using the checkerboard method, we confirmed the synergistic effect of MEPL from P. longifolia and ceftriaxone (2:1) for MRSA with a marked reduction of the MIC value of the ceftriaxone from 8000 mu g/mL to 1000 mu g/mL. Moreover, the combination of MEPL with ceftriaxone significantly (p < 0.05) inhibited the presence of the resistant mecA gene in the tested strain. The LC-ESI-MS/MS analysis identified compounds that were reported to exhibit antimicrobial activity. Conclusively, the MEPL extract, an important etiological agent for skin diseases, showed worthy synergistic antimicrobial action against MRSA bacteria, thus supporting the traditional use of P. longifolia.
| Item Type: | Article |
|---|---|
| Funders: | Research University Grants (RUI) from the Universiti Sains Malaysia, Malaysia [Grant No: 1001/CIPPM/8012229] |
| Uncontrolled Keywords: | Ceftriaxone; Methicillin-resistant Staphylococcus aureus; Polyalthia longifolia; Synergistic effect; mecA gene; Multiplex PCR; Gene expression; Skin diseases |
| Subjects: | R Medicine > RS Pharmacy and materia medica |
| Divisions: | Faculty of Dentistry |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 27 Nov 2024 04:04 |
| Last Modified: | 28 Nov 2024 03:53 |
| URI: | http://eprints.um.edu.my/id/eprint/38545 |
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