Simultaneous detection of Methicillin-resistant staphylococcus aureus, acinetobacter baumanii, escherichia coli, klebsiella pneumoniae and pseudomonas aeruginosa by multiplex PCR

Thong, K. L.; Lai, M.Y.; Teh, C.S.J.; Chua, K.H. (2011) Simultaneous detection of Methicillin-resistant staphylococcus aureus, acinetobacter baumanii, escherichia coli, klebsiella pneumoniae and pseudomonas aeruginosa by multiplex PCR. Trop Biomed, 28 (1). pp. 21-31. ISSN 0127-5720

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Abstract

A PCR-based assay that can simultaneously detect and differentiate five different types of nosocomial bacterial pathogens was developed. Six pairs of selected primers targeting femA (132 bp) and mecA (310 bp) of methicillin-resistant Staphylococcus aureus, gltA (722 bp) of Acinetobacter baumannii, phoA (903 bp) of Escherichia coli, mdh (364 bp) of Klebsiella pneumoniae and oprL (504 bp) of Pseudomonas aeruginosa were used in this study. The conditions were optimized for the multiplex PCR to ensure specific amplification of the selected targets. Sensitivity and specificity tests were also carried out using a blind test approach on 50 bacterial cultures and resulted in 100 for both positive and negative predictive values.

Item Type: Article
Creators:
  1. Thong, K. L.(Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
  2. Lai, M.Y.
  3. Teh, C.S.J.
  4. Chua, K.H.(Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
Journal or Publication Title: Trop Biomed
Additional Information: Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
Uncontrolled Keywords: Molecular Medicine
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms azrahani halim
Date Deposited: 01 Oct 2012 11:23
Last Modified: 22 Jan 2014 16:34
URI: http://eprints.um.edu.my/id/eprint/3848

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