Al-Trad, Esra'a I. and Che Hamzah, Ainal Mardziah and Puah, Suat Moi and Chua, Kek Heng and Hanifah, Muhamad Zarul and Ayub, Qasim and Palittapongarnpim, Prasit and Kwong, Stephen M. and Chew, Ching Hoong and Yeo, Chew Chieng (2023) Complete genome sequence and analysis of a ST573 multidrug-resistant methicillin-resistant staphylococcus aureus SauR3 clinical isolatE from Terengganu, Malaysia. Pathogens, 12 (3). ISSN 2076-0817, DOI https://doi.org/10.3390/pathogens12030502.
Full text not available from this repository.Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) is a World Health Organization-listed priority pathogen. Scarce genomic data are available for MRSA isolates from Malaysia. Here, we present the complete genome sequence of a multidrug-resistant MRSA strain SauR3, isolated from the blood of a 6-year-old patient hospitalized in Terengganu, Malaysia, in 2016. S. aureus SauR3 was resistant to five antimicrobial classes comprising nine antibiotics. The genome was sequenced on the Illumina and Oxford Nanopore platforms and hybrid assembly was performed to obtain its complete genome sequence. The SauR3 genome consists of a circular chromosome of 2,800,017 bp and three plasmids designated pSauR3-1 (42,928 bp), pSauR3-2 (3011 bp), and pSauR3-3 (2473 bp). SauR3 belongs to sequence type 573 (ST573), a rarely reported sequence type of the staphylococcal clonal complex 1 (CC1) lineage, and harbors a variant of the staphylococcal cassette chromosome mec (SCCmec) type V (5C2&5) element which also contains the aac(6 `)-aph(2 `') aminoglycoside-resistance genes. pSauR3-1 harbors several antibiotic resistance genes in a 14,095 bp genomic island (GI), previously reported in the chromosome of other staphylococci. pSauR3-2 is cryptic, whereas pSauR3-3 encodes the ermC gene that mediates inducible resistance to macrolide-lincosamide-streptogramin B (iMLS(B)). The SauR3 genome can potentially be used as a reference genome for other ST573 isolates.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Staphylococcus aureus ST573; Hybrid assembly; Resistance genes; SCCmec element; Plasmids; Genomic islands; Prophages |
| Subjects: | Q Science > QR Microbiology |
| Divisions: | Faculty of Medicine > Biomedical Science Department |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 26 Jun 2023 06:37 |
| Last Modified: | 26 Jun 2023 06:37 |
| URI: | http://eprints.um.edu.my/id/eprint/38477 |
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