Machain-Williams, Carlos and Reyes-Solis, Guadalupe. C. C. and Blitvich, Bradley. J. J. and Laredo-Tiscareno, Viridiana and Dzul-Rosado, Alma. R. R. and Kim, Sungmin and AbuBakar, Sazaly (2023) Evaluation of an immunoglobulin E capture enzyme-linked immunosorbent assay for the early diagnosis of dengue. Viral Immunology, 36 (2). pp. 101-109. ISSN 0882-8245, DOI https://doi.org/10.1089/vim.2022.0110.
Full text not available from this repository.Abstract
Dengue virus (DENV) is the etiological agent of dengue, the most important mosquito-transmitted viral disease of humans worldwide. Enzyme-linked immunosorbent assays (ELISAs) designed to detect DENV IgM are commonly used for dengue diagnosis. However, DENV IgM is not reliably detected until >= 4 days after illness onset. Reverse transcription-polymerase chain reaction (RT-PCR) can diagnose early dengue but requires specialized equipment, reagents, and trained personnel. Additional diagnostic tools are needed. Limited work has been performed to determine whether IgE-based assays can be used for the early detection of vector-borne viral diseases, including dengue. In this study, we determined the efficacy of a DENV IgE capture ELISA for the detection of early dengue. Sera were collected within the first 4 days of illness onset from 117 patients with laboratory-confirmed dengue, as determined by DENV-specific RT-PCR. The serotypes responsible for the infections were DENV-1 and DENV-2 (57 and 60 patients, respectively). Sera were also collected from 113 dengue-negative individuals with febrile illness of undetermined etiology and 30 healthy controls. The capture ELISA detected DENV IgE in 97 (82.9%) confirmed dengue patients and none of the healthy controls. There was a high false positivity rate (22.1%) among the febrile non-dengue patients. In conclusion, we provide evidence that IgE capture assays have the potential to be explored for early diagnosis of dengue, but further research is necessary to address the possible false positivity rate among patients with other febrile illnesses.
| Item Type: | Article |
|---|---|
| Funders: | Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MSIT) [Grant No: 2016M3A9B8942289 and C.M.-W. and S.K.], Ministry of Higher Education Malaysia, HICOE Program [Grant No: M0002-2019 (S.A.)] |
| Uncontrolled Keywords: | Infectious disease; Flavivirus; Dengue; IgE; ELISA; Diagnosis |
| Subjects: | Q Science > Q Science (General) Q Science > QR Microbiology |
| Divisions: | Deputy Vice Chancellor (Research & Innovation) Office > Tropical Infectious Diseases Research and Education Centre |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 10 Nov 2024 04:45 |
| Last Modified: | 10 Nov 2024 04:45 |
| URI: | http://eprints.um.edu.my/id/eprint/38467 |
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