Multiple-Locus Variable-Number Tandem Repeat Analysis of Vibrio cholerae in Comparison with Pulsed Field Gel Electrophoresis and Virulotyping

Teh, Cindy Shuan Ju and Chua, Kek Heng and Thong, Kwai Lin (2010) Multiple-Locus Variable-Number Tandem Repeat Analysis of Vibrio cholerae in Comparison with Pulsed Field Gel Electrophoresis and Virulotyping. Journal of Biomedicine and Biotechnology, 2010. p. 817190. ISSN 1110-7243,

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Abstract

Molecular analysis of Malaysian Vibrio cholerae was carried out using a multiple-locus variable-number tandem repeat analysis (MLVA) assay based on 7 loci of V. cholerae. The discriminatory ability of the assay was compared with pulsed-field gel electrophoresis (PFGE) using 43 Malaysian V. cholerae isolated from various sources. In addition, the virulotypes of the strains were determined. Based on MLVA, 38 allelic profiles were obtained (F=0.63) while PFGE generated 35 pulsotypes (F=0.71). Simpson's index of diversity for different VNTR loci ranged from 0.59 to 0.92. The combined loci increased the discriminatory index to 0.99 which was comparable with PFGE (D=0.99). Most of the environmental non-O1/non-O139 strains harbored rtxA, rstR, toxR, and hlyA only, and the virulotype of this serogroup was significantly different (P<.01) from clinical/environmental O1 and environmental O139 strains. In conclusion, the MLVA assay developed in this study was a useful genotyping tool with comparable discriminatory power with PFGE. In addition, the combination of the two approaches can further distinguish the strains from different sources and geographical regions of isolation.

Item Type:	Article
Funders:	University Malaya Research Grant (RG017-09BIO), MTSF Science and Technology Grant (MTSF1051-2009A), National Institute of Infectious Disease (Japan) grant
Additional Information:	Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
Uncontrolled Keywords:	Molecular Medicine; Vibrio cholerae
Subjects:	Q Science > Q Science (General) Q Science > QH Natural history R Medicine
Divisions:	Faculty of Medicine Faculty of Science > Institute of Biological Sciences
Depositing User:	Ms azrahani halim
Date Deposited:	02 Nov 2012 01:15
Last Modified:	26 Feb 2019 08:25
URI:	http://eprints.um.edu.my/id/eprint/3840

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