Molecular investigation of virulence determinants between a virulent clinical strain and an attenuated strain of burkholderia pseudomallei

Puthucheary, S.D.; Puah, S.M.; Chai, H.C.; Thong, K. L.; Chua, K.H. (2012) Molecular investigation of virulence determinants between a virulent clinical strain and an attenuated strain of burkholderia pseudomallei. Journal of Molecular Microbiology and Biotechnology, 22 (3). pp. 198-204. ISSN 1464-1801

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Abstract

Burkholderia pseudomallei is the causative agent of melioidosis. We initiated this investigation with a virulent and an attenuated strain of B. pseudomallei. Pulsed-field gel electrophoresis was carried out initially for macrogenomic comparison of both strains of B. pseudomallei. However, the pulsotypes obtained were identical and therefore we applied a subtractive hybridization technique to distinguish and determine the possible differences between the two strains. Six virulence strain-specific DNA fragments were obtained and the encoding homolog proteins were identified as a xenobiotic-responsive element family of transcriptional regulator, a hypothetical protein, an unknown protein, a plasmid recombination enzyme, a regulatory protein and a putative hemolysin activator protein. A combination of at least three of these determinants was identified in 45 clinical isolates when screening was carried out with self-designed multiplex PCR targeting the six putative virulent determinants. Our data demonstrated that different combinations of the six putative virulence genes were present in the clinical isolates indicating their probable role in the pathogenesis of B. pseudomallei infections.

Item Type: Article
Creators:
  1. Puthucheary, S.D.
  2. Puah, S.M.
  3. Chai, H.C.
  4. Thong, K. L.(Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
  5. Chua, K.H.(Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
Journal or Publication Title: Journal of Molecular Microbiology and Biotechnology
Additional Information: Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
Uncontrolled Keywords: Burkholderia pseudomallei, Subtractive hybridization, Virulence determinants
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms azrahani halim
Date Deposited: 01 Nov 2012 16:18
Last Modified: 22 Jan 2014 16:26
URI: http://eprints.um.edu.my/id/eprint/3839

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