Lai, Meng Yee and Zen, Lee Phone Youth and Hamid, Mohd Hafizi Abdul and Jelip, Jenarun and Mudin, Rose Nani and Ivan, Vun Jan Shui and Francis, Lee Ngie Ping and Saihidi, Izreena and Lau, Yee Ling (2023) Point-of-care diagnosis of malaria using a simple, purification-free DNA extraction method coupled with loop-mediated isothermal amplification-lateral flow. Tropical Medicine and Infectious Disease, 8 (4). ISSN 2414-6366, DOI https://doi.org/10.3390/tropicalmed8040199.
Full text not available from this repository.Abstract
We propose a protocol suitable for point-of-care diagnosis of malaria utilizing a simple and purification-free DNA extraction method with the combination of loop-mediated isothermal amplification assay and lateral flow (LAMP-LF). The multiplex LAMP-LF platform developed here can simultaneously detect Plasmodium knowlesi, P. vivax, P. falciparum, and Plasmodium genus (for P. malariae and P. ovale). Through the capillary effect, the results can be observed by the red band signal on the test and control lines within 5 min. The developed multiplex LAMP-LF was tested with 86 clinical blood samples on-site at Hospital Kapit, Sarawak, Malaysia. By using microscopy as the reference method, the multiplex LAMP-LF showed 100% sensitivity (95% confidence interval (CI): 91.4 to 100.00%) and 97.8% specificity (95% CI: 88.2% to 99.9%). The high sensitivity and specificity of multiplex LAMP-LF make it ideal for use as a point-of-care diagnostic tool. The simple and purification-free DNA extraction protocol can be employed as an alternative DNA extraction method for malaria diagnosis in resource-limited settings. By combining the simple DNA extraction protocol and multiplex LAMP-LF approach, we aim to develop a simple-to-handle and easy-to-read molecular diagnostic tool for malaria in both laboratory and on-site settings.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Higher Education, Malaysia [Grant No: LRGS/1/2018/UM/01/1/4 (LR002D-2018)] |
| Uncontrolled Keywords: | Malaria; DNA extraction; LAMP; Molecular diagnosis; Filter paper-based; Lateral flow; Point-of-care |
| Subjects: | R Medicine > RC Internal medicine R Medicine > RX Homeopathy |
| Divisions: | Faculty of Medicine > Parasitology Deparment |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 08 Nov 2024 02:51 |
| Last Modified: | 08 Nov 2024 02:51 |
| URI: | http://eprints.um.edu.my/id/eprint/38344 |
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