Al-Trad, Esra'a I. and Chew, Ching Hoong and Hamzah, Ainal Mardziah Che and Suhaili, Zarizal and Rahman, Nor Iza A. and Ismail, Salwani and Puah, Suat Moi and Chua, Kek Heng and Kwong, Stephen M. and Yeo, Chew Chieng (2023) The plasmidomic landscape of clinical methicillin-resistant staphylococcus aureus isolates from Malaysia. Antibiotics-Basel, 12 (4). ISSN 2079-6382, DOI https://doi.org/10.3390/antibiotics12040733.
Full text not available from this repository.Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) is a priority nosocomial pathogen with plasmids playing a crucial role in its genetic adaptability, particularly in the acquisition and spread of antimicrobial resistance. In this study, the genome sequences of 79 MSRA clinical isolates from Terengganu, Malaysia, (obtained between 2016 and 2020) along with an additional 15 Malaysian MRSA genomes from GenBank were analyzed for their plasmid content. The majority (90%, 85/94) of the Malaysian MRSA isolates harbored 1-4 plasmids each. In total, 189 plasmid sequences were identified ranging in size from 2.3 kb to ca. 58 kb, spanning all seven distinctive plasmid replication initiator (replicase) types. Resistance genes (either to antimicrobials, heavy metals, and/or biocides) were found in 74% (140/189) of these plasmids. Small plasmids (<5 kb) were predominant (63.5%, 120/189) with a RepL replicase plasmid harboring the ermC gene that confers resistance to macrolides, lincosamides, and streptogramin B (MLSB) identified in 63 MRSA isolates. A low carriage of conjugative plasmids was observed (n = 2), but the majority (64.5%, 122/189) of the non-conjugative plasmids have mobilizable potential. The results obtained enabled us to gain a rare view of the plasmidomic landscape of Malaysian MRSA isolates and reinforces their importance in the evolution of this pathogen.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Multidrug Resistant MRSA; Plasmids; Plasmid mobilization; Replicon; Resistance; Whole genome sequencing |
| Subjects: | R Medicine R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine |
| Divisions: | Faculty of Medicine Faculty of Medicine > Biomedical Science Department |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 01 Dec 2023 13:36 |
| Last Modified: | 01 Dec 2023 13:36 |
| URI: | http://eprints.um.edu.my/id/eprint/38325 |
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