Investigation of SLC2A1 26177A/G gene polymorphism via high resolution melting curve analysis in Malaysian patients with diabetic retinopathy

Ng, Z.X.; Kuppusamy, U.R.; Tajunisah, I.; Fong, K.C.S.; Chua, K.H. (2012) Investigation of SLC2A1 26177A/G gene polymorphism via high resolution melting curve analysis in Malaysian patients with diabetic retinopathy. Journal of Diabetes and its Complications, 26 (5). pp. 388-392. ISSN 1056-8727

[img] PDF - Published Version
Restricted to Registered users only [error in script]

Download (704Kb) | Request a copy

    Abstract

    Purpose In this study, we aimed to investigate the possible association between SLC2A126177A/Gpolymorphism and diabetic retinopathy (DR) in Malaysianpatients with type 2 diabetes. Methods Genomic DNA was extracted from 211 Malaysian type 2 diabeticpatients (100 without retinopathy DNR, 111 with retinopathy) and 165 healthy controls. A highresolutionmelting assay developed in this study was used to detect SLC2A126177A/Gpolymorphism followed by statistical analysis. Results A statistically significant difference in 26177 G minor allele frequency between healthy controls (19.7 %) and total patient group (26.1 %) (p < 0.05, Odd ratio = 1.437, 95% Confidence interval = 1.015�2.035) as well as between healthy controls (19.7 %) and DNR patients (27.5%) (p < 0.05, Odd ratio = 1.546, 95% Confidence interval = 1.024�2.336) was shown in this study. However, when compared between DR and DNR patients, there was no significant difference (p > 0.05). Conclusions This is the first study which shows that SLC2A1 26177G allele is associated with type 2 diabetes in Malaysian population but not with DR.

    Item Type: Article
    Creators:
    1. Ng, Z.X.
    2. Kuppusamy, U.R.
    3. Tajunisah, I.
    4. Fong, K.C.S.
    5. Chua, K.H.(Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    Journal or Publication Title: Journal of Diabetes and its Complications
    Additional Information: Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
    Uncontrolled Keywords: Diabetic retinopathy; GLUT1; HRM; Polymorphism; SLC2A1
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Ms azrahani halim
    Date Deposited: 02 Nov 2012 09:24
    Last Modified: 02 Nov 2012 09:24
    URI: http://eprints.um.edu.my/id/eprint/3831

    Actions (For repository staff only: Login required)

    View Item

    Document Downloads

    More statistics for this item...