Identification of Burkholderia pseudomallei Mimotope using Phage display approach

Chua, K.H.; Chai, H.C.; Puthucheary, S.D. (2008) Identification of Burkholderia pseudomallei Mimotope using Phage display approach. Journal of Applied Sciences, 8 (21). pp. 3999-4003. ISSN 1812-5654

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    Abstract

    This study reportsa sequential biopanning against serum obtained from a patient infected with B. pseudomallei using a phage display peptide library. A peptide bearing the consensus sequence NKNSFDAWLQSF was obtained after 3 rounds of biopanning and it partly resembles the sequence 35FDAWLAAQEF44 of B. pseudomallei site-specific recombinase, which is a phage integrase family protein. Overall, the mimotope obtained in this study could be a potential candidate for the development of a cost effective diagnostic test or vaccine in the future.

    Item Type: Article
    Creators:
    1. Chua, K.H.(Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSI)
    2. Chai, H.C.
    3. Puthucheary, S.D.
    Journal or Publication Title: Journal of Applied Sciences
    Additional Information: Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSI
    Uncontrolled Keywords: Burkholderia pseudomallei ; melioidosis ; phage display ; mimotope
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Ms azrahani halim
    Date Deposited: 01 Nov 2012 12:22
    Last Modified: 01 Nov 2012 12:22
    URI: http://eprints.um.edu.my/id/eprint/3822

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