Identification of Burkholderia pseudomallei Mimotope using Phage display approach

Chua, K.H. and Chai, H.C. and Puthucheary, S.D. (2008) Identification of Burkholderia pseudomallei Mimotope using Phage display approach. Journal of Applied Sciences, 8 (21). pp. 3999-4003. ISSN 1812-5654,

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Abstract

This study reportsa sequential biopanning against serum obtained from a patient infected with B. pseudomallei using a phage display peptide library. A peptide bearing the consensus sequence NKNSFDAWLQSF was obtained after 3 rounds of biopanning and it partly resembles the sequence 35FDAWLAAQEF44 of B. pseudomallei site-specific recombinase, which is a phage integrase family protein. Overall, the mimotope obtained in this study could be a potential candidate for the development of a cost effective diagnostic test or vaccine in the future.

Item Type: Article
Funders: UNSPECIFIED
Additional Information: Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSI
Uncontrolled Keywords: Burkholderia pseudomallei ; melioidosis ; phage display ; mimotope
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms azrahani halim
Date Deposited: 01 Nov 2012 04:22
Last Modified: 01 Nov 2012 04:22
URI: http://eprints.um.edu.my/id/eprint/3822

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