Bioinformatics in molecular immunology laboratories demonstrated: modeling an anti-CMV scFv antibody

Chua, K.H.; Othman, R.Y. (2006) Bioinformatics in molecular immunology laboratories demonstrated: modeling an anti-CMV scFv antibody. Bioinformation, 1 (4). 118 - 120. ISSN 0973-2063

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    Abstract

    A scFv (single chain variable fragment) antibody clone from anti-CMV (anti-cucumber mosaic virus) was successfully constructed from immunized mouse and the DNA sequence was submitted to GenBank (AY337618 and AY337619). The expression of a 32 kDa recombinant antibody in bacteria was verified using ELISA (enzyme-linked immunoassay) and western blot. However, elucidation of specific anti-CMV scFv function requires detailed and time consuming immuno-assays. Alternatively, useful functional information on anti-CMV scFV antibody can be obtained using available Bioinformatics tools and techniques without performing tedious assays. Here, we use the commonly used Bioinformatics tools and databases such as BLAST (basic local alignment search tool), GenBank, PDB (protein databank), KABAT numbering, SWISS-MODEL and Insight II to gain specific functional insights into anti-CMV scFv.

    Item Type: Article
    Creators:
    1. Chua, K.H.(Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    2. Othman, R.Y.
    Journal or Publication Title: Bioinformation
    Additional Information: Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
    Uncontrolled Keywords: anti-CMV scFv, sequencing, GenBank, homology modeling, CDR, epitopes
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Ms azrahani halim
    Date Deposited: 01 Nov 2012 12:28
    Last Modified: 01 Nov 2012 12:28
    URI: http://eprints.um.edu.my/id/eprint/3787

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