2245G/A polymorphism of the receptor for advanced glycation end-products (RAGE) gene is associated with diabetic retinopathy in the Malaysian population

Ng, Z.X.; Kuppusamy, U.R.; Tajunisah, I.; Fong, K.C.S.; Koay, A.C.A.; Chua, K.H. (2012) 2245G/A polymorphism of the receptor for advanced glycation end-products (RAGE) gene is associated with diabetic retinopathy in the Malaysian population. The British Journal of Ophthalmology, 96 (2). pp. 289-292.

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    Abstract

    Background/aims The receptor for advanced glycation end-products (RAGE) has been implicated in the pathogenesis of diabetic microvascular complications. The aim of this study was to investigate the association between 2245G/A gene polymorphism of the RAGE gene and retinopathy in Malaysian type 2 diabetic patients. Methods 342 unrelated type 2 diabetic patients (171 with retinopathy (DR), 171 without retinopathy (DNR)) and 235 unrelated healthy subjects from all over Malaysia were recruited for this study. Genomic DNA was isolated from 3�ml samples of whole blood using a modified conventional DNA extraction method. The genotype and allele frequencies of 2245G/A were studied using the polymerase chain reaction�restriction fragment length polymorphism (PCR-RFLP) method. Results A statistically significant difference in 2245A minor allele frequency was found between control (5.5) and DR groups (15.2) (p<0.001, OR=3.06, 95 CI 1.87 to 5.02) as well as between DNR (8.2) and DR (15.2) groups (p<0.01, OR=2.01, 95 CI 1.24 to 3.27). However, when the frequency was compared between control and DNR groups, there was no significant difference (p>0.05). Conclusions This is the first study that shows an association between the 2245A allele of the RAGE gene and development of diabetic retinopathy in the Malaysian population.

    Item Type: Article
    Journal or Publication Title: The British Journal of Ophthalmology
    Additional Information: Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA ---> Correspondence to Professor Kek Heng Chua, Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia; khchua@um.edu.my
    Uncontrolled Keywords: Molecular Medicine
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Ms azrahani halim
    Date Deposited: 25 Oct 2012 10:06
    Last Modified: 25 Oct 2012 10:06
    URI: http://eprints.um.edu.my/id/eprint/3779

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