Ng, Zhi Xiang and Kuppusamy, Umah Rani and Tajunisah, Iqbal and Fong, Kenneth Choong Sian and Koay, Adrian Choon Aun and Chua, Kek Heng (2012) 2245G/A polymorphism of the receptor for advanced glycation end-products (RAGE) gene is associated with diabetic retinopathy in the Malaysian population. British Journal of Ophthalmology, 96 (2). pp. 289-292. ISSN 0007-1161, DOI https://doi.org/10.1136/bjophthalmol-2011-300658.
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2245G-A_polymorphism_of_the_receptor_for_advanced_glycation_end-products_(RAGE)_gene_is_associated_with_diabetic_retinopathy_in_the_Malaysian_population.pdf - Published Version Download (119kB) |
Abstract
Background/aims The receptor for advanced glycation end-products (RAGE) has been implicated in the pathogenesis of diabetic microvascular complications. The aim of this study was to investigate the association between 2245G/A gene polymorphism of the RAGE gene and retinopathy in Malaysian type 2 diabetic patients. Methods 342 unrelated type 2 diabetic patients (171 with retinopathy (DR), 171 without retinopathy (DNR)) and 235 unrelated healthy subjects from all over Malaysia were recruited for this study. Genomic DNA was isolated from 3�ml samples of whole blood using a modified conventional DNA extraction method. The genotype and allele frequencies of 2245G/A were studied using the polymerase chain reaction�restriction fragment length polymorphism (PCR-RFLP) method. Results A statistically significant difference in 2245A minor allele frequency was found between control (5.5) and DR groups (15.2) (p<0.001, OR=3.06, 95 CI 1.87 to 5.02) as well as between DNR (8.2) and DR (15.2) groups (p<0.01, OR=2.01, 95 CI 1.24 to 3.27). However, when the frequency was compared between control and DNR groups, there was no significant difference (p>0.05). Conclusions This is the first study that shows an association between the 2245A allele of the RAGE gene and development of diabetic retinopathy in the Malaysian population.
Item Type: | Article |
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Funders: | UNSPECIFIED |
Additional Information: | Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA ---> Correspondence to Professor Kek Heng Chua, Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia; khchua@um.edu.my |
Uncontrolled Keywords: | Molecular Medicine |
Subjects: | R Medicine |
Divisions: | Faculty of Medicine |
Depositing User: | Ms azrahani halim |
Date Deposited: | 25 Oct 2012 02:06 |
Last Modified: | 02 Aug 2019 05:57 |
URI: | http://eprints.um.edu.my/id/eprint/3779 |
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