Bacteriostatic effect of piper betle and psidium guajava extracts on dental plaque bacteria

Fathilah, A.R.; Rahim, Z.H.A.; Othman, Y.; Yusoff, M. (2009) Bacteriostatic effect of piper betle and psidium guajava extracts on dental plaque bacteria. Pakistan Journal of Biological Sciences, 12 (6). pp. 518-521. ISSN 10288880

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    Abstract

    In this study, the bacteriostatic effect of Piper betle and Psidium guajava extracts on selected early dental plaque bacteria was investigated based on changes in the doubling time (g) and specific growth rates (μ). Streptococcus sanguinis, Streptococcus mitis and Actinomyces sp. were cultured in Brain Heart Infusion (BHI) in the presence and absence of the extracts. The growth of bacteria was monitored periodically every 15 min over a period of 9 h to allow for a complete growth cycle. Growth profiles of the bacteria in the presence of the extracts were compared to those in the absence and deviation in the g and μ were determined and analyzed. It was found that the g and μ were affected by both extracts. At 4 mg mL-1 of P. betle the g-values for 5. sanguinis and 5. mitis were increased by 12.0- and 10.4-fold, respectively (p<0.05). At similar concentration P. guajava increased the g-value by 1.8- and 2.6-fold, respectively (p<0.05). The effect on Actinomyces sp. was observed at a much lower magnitude. It appears that P, betle and P, guajava extracts have bacteriostatic effect on the plaque bacteria by creating a stressed environment that had suppressed the growth and propagation of the cells. Within the context of the dental plaque, this would ensure the attainment of thin and healthy plaque. Thus, decoctions of these plants would be suitable if used in the control of dental plaque. © 2009 Asian Network for Scientific Information.

    Item Type: Article
    Creators:
    1. Fathilah, A.R.
    2. Rahim, Z.H.A.
    3. Othman, Y.
    4. Yusoff, M.
    Journal or Publication Title: Pakistan Journal of Biological Sciences
    Additional Information: Cited By (since 1996): 5 Export Date: 10 September 2012 Source: Scopus PubMed ID: 19580002 Language of Original Document: English Correspondence Address: Fathilah, A. R.; Department of Oral Biology, Faculty of Dentistry, 50603 Kuala Lumpur, Malaysia Chemicals/CAS: Anti-Bacterial Agents; Plant Extracts References: Baehni, P.C., Takeuchi, Y., Anti-plaque agents in the prevention of biofilm-associated oral disease (2003) Oral Dis, 9, pp. 23-29; Cappuccino, J.G., Sherman, N., Microbiology-A laboratory manual (2005) Pearson Int, , 7th Edn, Ontario, Canada; Cummins, D., Mechanisms of Action of Clinically Proven Anti-Plaque Agents (1992) Clinical and Biological Aspects of Dentifrices, pp. 205-228. , Embery, G. and G. Rolla Eds, Oxford University Press, London, pp; Fathilah, A.R., Rahim, Z.H.A., The anti-adherence effect of Piper betle and Psidium guajava extracts on the adhesion of early settlers in dental plaque to saliva-coated glass surfaces (2003) J. Oral Sci, 45, pp. 201-206; Fathilah, A.R., Othman, Y., Rahim, Z.H.A., The effect of Piper betle and Psidium guajava extracts on the cell-surface hydrophobicity of selected early settlers of dental plaque (2006) J. Oral Sci, 48, pp. 71-75; Fathilah, A.R., Aishah, A., Zarina, M.Z., The effect of environmental stress on the growth of plaque bacteria (2007) J. Microbiol, 4, pp. 381-386; Gerhardt, P., Murray, R.G.E., Costlow, R.N., Nester, E.W., Wood, W.A., (1981) Manual of Methods for General Bacteriology, , 1st Edn, American Society for Microbiology, Washington, DC; Harper, D.S., Mueller, L.F., Fine, J.B., Gordon, J., Laster, L.L., Effect of 6 months use of a dentifrice and oral rinse containing sanguinarine extract and zinc chloride upon the microflora of the dental plaque and oral soft tissues (1990) J. Periodontol, 61, pp. 359-363; Hirasawa, M., Takada, K., Otake, S., Inhibition of acid production in dental plaque bacteria by green tea catechins (2006) Caries Res, 40, pp. 265-270; Jones, C.G., Chlorhexidine: Is it still the gold standard? (1997) Periodontology, 15, pp. 55-62; Kamath, J.V., Rahul, N., Kumar, C.K.A., Lakshmi, S.M., Psidium guajava L.: A review (2008) Int. J. Green Pharm, 2, pp. 9-12; Kopczyk, R., Abrams, J., Brown, A., Matheny, J., Kaplan, A., Clinical and microbiological effects of a sanguinarine-containing mouth rinse and dentifrice with and without fluoride during 6 months of use (1991) J. Periodontol, 62, pp. 617-622; Kornman, K.S., Antimicrobial Agents (1986) Dental Plaque Control Measures and Oral Hygiene Practice, pp. 121-142. , Loe, H. and D. Kleiman, Eds, Oxford University Press, USA, pp; Nair, R., Chanda, S., Antimicrobial activity of Terminalia catappa, Manilkara zapota and Piper betel leaf (2008) Indian J. Pharm. Sci, 70, pp. 390-393; Nalina, T., Rahim, Z.H.A., Effect of Piper betle L. leaf extract on the virulence activity of Streptococcus mutans: An in vitro study (2006) Pak. J. Biol. Sci, 9, pp. 1470-1475; Nalina, T., Rahim, Z.H.A., The crude aqueous extract of Piper betle L. and its antibacterial effect towards Streptococcus mutans (2007) Am. J. Biotechnol. Biochem, 3, pp. 10-15; Otake, S., Makimura, M., Kuroki, T., Nishihara, Y., Hirasawa, M., Anticaries effects of polyphenolic compounds from Japanese green tea (1991) Caries Res, 25, pp. 438-443; Ponglux, D., Wong, S., Phadungcharoen, T., Ruangrungsri, N., Likhitwitayawuid, K., (1987) Medicinal Plants, , 1st Edn, Victory Power Point Corp Ltd, Bangkok, Thailand; Wirotesangthong, M., Inagaki, N., Tanaka, H., Thanakijcharoenpath, W., Nagai, H., Inhibitory effects of Piper betle on production of allergic mediators by bone marrow-derived mast cells and lung epithelial cells (2008) Int. Immunopharm, 8, pp. 453-457; Wollinsky, L.E., Mama, S., Nachnani, S., Ling, S., The inhibiting effect of aqueous Azaradichta indica (Neem) extract upon bacterial properties influencing in vitro plaque formation (1996) J. Dent. Res, 75, pp. 816-822; Wu-Yuan, C.D., Chen, C.Y., Wu, R.T., Gallotannins inhibit growth, water-insoluble glucan synthesis and aggregation of mutans streptococci (1988) J. Dent. Res, 67, pp. 51-55
    Uncontrolled Keywords: Bacteriostatic effect Dental plaque bacteria Piper betle Psidium guajava antiinfective agent plant extract article bacterium chemistry drug effect guava histology human microbiological examination microbiology plant leaf tooth plaque Anti-Bacterial Agents Bacteria Dental Plaque Humans Microbial Sensitivity Tests Plant Extracts Plant Leaves Psidium Actinomyces Actinomyces sp. Bacteria (microorganisms) Streptococcus mitis Streptococcus sanguinis
    Subjects: R Medicine > RK Dentistry
    Divisions: Faculty of Dentistry > Dept of Oral Biology
    Depositing User: Mr Ahmad Azwan Azman
    Date Deposited: 23 Oct 2012 11:51
    Last Modified: 23 Oct 2012 11:51
    URI: http://eprints.um.edu.my/id/eprint/3742

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