Chin, Yao Xian and Chen, Xin and Cao, Wan Xiu and Sharifuddin, Yusrizam and Green, Brian D. and Lim, Phaik Eem and Xue, Chang Hu and Tang, Qing Juan (2020) Characterization of seaweed hypoglycemic property with integration of virtual screening for identification of bioactive compounds. Journal of Functional Foods, 64. ISSN 1756-4646, DOI https://doi.org/10.1016/j.jff.2019.103656.
Full text not available from this repository.Abstract
Seaweeds have been studied extensively for their nutritional values but their potential nutraceutical application remained underutilized due to uncharacterized bioactive compounds. Here we demonstrated that water extracts of Kappaphycus, Halimeda, Padina and Sargassum were able to improve insulin resistance, reduced hyperglycemia and protect liver and pancreatic tissue from HFD-induced damage in mice, with both Padina and Sargasssum displayed more significant results than the other two seaweeds. A list of potential bioactive compounds was then composed by virtual screening of 276 compounds detected by LC-MS on selected Padina fractions using molecular docking by Surflex-Dock. Further analysis determined punicate as the most potent bioactive compound that inhibits both glucosidase and dipeptidyl-peptidase-4 enzymes. In conclusion, we discovered novel in vivo hypoglycemic activity in Halimeda and several potential alpha-glucosidase and DPP-4 inhibitors in Padina via virtual screening, demonstrating the efficacy of molecular docking to facilitate discovery of novel bioactive compounds.
| Item Type: | Article |
|---|---|
| Funders: | Ocean University of China via National Key R&D Program of China (2018YFC0311201), Universiti Malaya (RG109-11SUS), Universiti Malaya (UMQUB2A-2011) |
| Uncontrolled Keywords: | Bioactive compound; Hypoglycaemia; Seaweed; Virtual screening; Molecular docking; Surflex-Dock |
| Subjects: | Q Science > Q Science (General) |
| Divisions: | Faculty of Science > Institute of Biological Sciences Deputy Vice Chancellor (Research & Innovation) Office > Institute of Ocean and Earth Sciences |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 16 Jun 2023 06:51 |
| Last Modified: | 16 Jun 2023 06:51 |
| URI: | http://eprints.um.edu.my/id/eprint/37000 |
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